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娜托百合的组织培养和离体快繁 被引量:4

Study on tissue culture and propagation of nettuno
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摘要 以娜托百合的鳞茎及幼茎为外植体成功获得再生植株,并建立了快速无性繁殖系.外植体的最佳芽诱导培养基是MS+0 2~0 5mg/LNAA+0 05~0 8mg/LBA;MS+0 2~0 5mg/LNAA+0 8~2 0mg/LKT;芽的最佳增殖培养基为MS+0 05~0 4mg/LIAA+0 05~0 4mg/LKT;MS+0 1~0 5mg/LBA+0 05~0 5mg/LNAA;芽的最佳增壮培养基和根诱导最佳培养基为MS+0 05~0 8mg/LNAA+0 05~1 0mg/LKT. The clonal propagation of nettuno is developed through culture of scales and stem segments in vitro.Better induction medium of shoots are MS+0.2~0.5 mg/L NAA+0.05~0.8 mg/L BA; MS+0.2~0.5 mg/L NAA+0.8~2.0 mg/L KT;Proliferation medium of shoots are MS+0.05~0.4 mg/L IAA+0.05~0.4 mg/L KT; MS+0.1~0.5 mg/L BA+0.05~0.5 mg/L NAA. The sturdiness of shoots and induction of roots are better in medium of MS+0.05~0.8 mg/L NAA+0.05~1.0 mg/L KT.
出处 《西北师范大学学报(自然科学版)》 CAS 2004年第4期74-76,共3页 Journal of Northwest Normal University(Natural Science)
基金 甘肃省计划委员会基金资助项目(5002/126) 国家星火计划资助项目(2001EA86005)
关键词 娜托百合 组织培养 无性繁殖系 nettuno tissure culture clonal propagation
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