摘要
采用基因测序的方法,对我国海南产的羊鲍和耳鲍两个自然种群的COⅠ和COⅡ基因片段进行了PCR扩增和测序。结果表明,羊鲍和耳鲍COⅠ基因片段的核苷酸序列均为193bp,4种碱基组成非常相似,且其A+T的含量也非常相似,分别为45.08%和45.60%。羊鲍和耳鲍COⅠ基因片段的核苷酸序列之间有29bp的差异,其中有8处发生碱基颠换,21处发生碱基转换,差异为15.03%,同源性为84.97%;羊鲍和耳鲍COⅡ基因片段的核苷酸序列均为157bp,羊鲍和耳鲍4种碱基组成差异较大,且其A+T的含量也不同,分别为59.24%和55.41%。羊鲍和耳鲍COⅡ基因片段的核苷酸序列之间有19bp的差异,其中3处发生碱基颠换,16处发生碱基转换,达到12.10%的差异,同源性为87.90%。分子变异分析表明羊鲍和耳鲍物种间的变异组成为0.50,变异百分数为100.00,羊鲍和耳鲍物种间的遗传分化显著(FST=1,P<0.001)。
MtDNA CO Ⅰ and CO Ⅱ gene fragments of Haliotis ovina and H. asinina were amplified by PCR, and the PCR products were purified and sequenced. The results showed that all the MtDNA CO Ⅰ gene fragments were 193bp for nucleotide sequences with similar base composition in the content of A＋T at 45.08% and 45.60% respectively. The base difference between the two was 29bp, including 8 base transversions and 21 base transitions, and the homology between them was 84.97%. All the MtDNA CO Ⅱ gene fragments of H. ovina and H. asinina were 157bp for nucleotide sequences, and the difference in base composition was large, so was the content of A＋T at 59.24% and 55.41% respectively. The base difference between the two species was 19, including 3 base transversions and 16 base transitions in homology of 87.90%. AMOVA analysis revealed significant genetic difference （FST = 1, P〈0.001）, and the use of the gene fragments is recommended for differentiating Haliotis ovina from H. asinina.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2008年第2期168-173,共6页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金资助项目,30271013号
福建省自然科学基金资助项目,B0110036号
国家留学回国人员科研启动基金,教外司留(2006)331号
集美大学创新团队基金资助,集大人(2007)17号
