摘要
应用RAPD标记技术对皱纹盘鲍杂交家系JC的双亲及93个F1个体进行了分析,对JC家系在皱纹盘鲍遗传连锁图谱构建中的应用前景进行了初步评估,并分析了RAPD分子标记在JC家系F1代中的分离方式及该家系的1对亲本及其子代个体的遗传结构.7条随机引物共扩增出41个分子标记,其中24个为多态性,占总数的58 5%;有14个分离标记可用于遗传连锁作图,有7个符合孟德尔独立分配定律,占总数的17 1%.2个标记与子代个体大小有一定的相关性,但其连锁关系仍有待于进一步研究.父母本之间的遗传距离为0 3844,父本与子代间的距离为0 1372,母本与子代间的距离为0 1273;父本提供的分离标记在子代中的显性频率为0 497,母本为0 595.双亲之间的遗传距离较远,杂交产生的JC-F1群体具有较高的杂合度(0 2016),该结果初步表明皱纹盘鲍杂交家系JC的子1代即可作为作图群体应用于皱纹盘鲍的连锁图谱构建.
The genetic structure and segregation of molecular markers in a F_1 hybrid family of Pacific abalone (Haliotis discus hannai Ino) were studied using random amplified polymorphic DNA (RAPD) technology. This family's usefulness in linkage mapping of abalones was assessed. Seven primers yielded a total of 41 markers, of which 24 (58.5%) were polymorphic in this family, 14 were informative and could be used for linkage mapping, and 7 (17.1%) markers were segregated in the expected Mendelian ratio which is very low compared with other species. Two markers show some association with JC-F_1's phenotypes, and this relationship needs further study. The genetic distances between two parents, father and progeny, mother and progeny are 0.384 4, 0.137 2, 0.127 3 respectively. The frequency in JC-F_1 population of RAPD markers from father and mother are 0.824 and 0.841, which are almost the same. High genetic distance between parents and high heterozygosity(0.201 6) in JC-F_1 population is also confirmd the feasibility of linkage mapping using these parents and progenies.
出处
《海洋学报》
CAS
CSCD
北大核心
2004年第6期124-132,共9页
基金
国家"863"计划课题资助项目(2002AA629030
2001AA621070)
国家自然科学基金资助项目(30371117
39825121).
关键词
皱纹盘鲍
RAPD
遗传结构
分子标记
连锁图谱
Haliotis discus hannai
RAPD
genetic structure
molecular markers
linkage mapping
