摘要
目的评价不同剂量凉血活血方对大鼠放射性肺损伤的防治作用。方法采用多次重复照射右肺的方法建立大鼠放射性肺损伤模型。72只Wistar大鼠被随机分为单纯照射组和凉血活血方大、中、小剂量组,分批处死大鼠,对各组大鼠的生物学特征、肺脏外观及组织病理进行观察和比较。结果凉血活血方大、中剂量组大鼠鼻周红赤、大便干硬等表现轻于小剂量组及单纯照射组;凉血活血方大、中、小剂量组除放射后26周时肺系数与单纯照射组差异无统计学意义外,其右肺湿重和肺系数在各时间点均明显低于单纯照射组,且照射5周时凉血活血方大、中剂量组右肺湿重亦明显低于小剂量组,差异有统计学意义(P均<0.01)。组织病理学观察显示,凉血活血方大、中剂量组肺脏表面肿胀、出血,肺间质充血、水肿以及肺泡结构破坏程度等也明显轻于小剂量组及单纯照射组。结论凉血活血方能减轻早期放射性肺损伤的炎症反应,减轻放射性肺纤维化的程度;而且凉血活血方大、中剂量组的防治效果明显好于小剂量组和单纯照射组。
Objective To evaluate the therapeutic effects and dose-effect relationships of different doses of the blood-cooling and blood flow-promoting drugs（凉血活血方）on radiation-induced lung injury of rat. Methods Seventy-two Wistar rats were randomly allocated into four groups： irradiation group （group A）, small-dose group （group B, 9g·kg^-1·d^-l）, middle-dose group （group C, 18g·kg^-1·d^-1） and high-dose group （group D, 36g ·kg^-1·d^-l）. All groups were repeatedly exposed to small dose of X-ray in the right hemi-thorax, and then the mice were sacrificed at different time points. The living animal features, the macro-changes of lung were observed, and pulmonary histopathological changes in all the groups were investigated, and the results of observation were compared. Results The symptoms of red patches around the nose and dry stool in groups C and D were less than those of groups A and B. After 26 weeks, lung coefficient in groups B, C and D was the same as that in group A, but right lung wet weights and lung coefficients at each time point in groups B, C and D were significantly lower than those in group A, and during 5th week, the right lung wet weights in groups C and D were obviously lower than the weight in group B （all P〈0. 01）, the macro-changes and histopathological observations indicated that the swelling appearance and hemorrhage at the lung surface, lung interstitial congestion and edema, and the destruction of alveoli in groups C and D were lower than those in groups A and B. Conclusion Using blood-cooling and blood flow-promoting drugs may ameliorate the early radiation-induced inflammatory reaction of lung injury, and decrease the severity of radioactive pulmonary fibrosis; the therapeutic effects in the middle-dose and high-dose groups are much better than those in the small-dose and simple radiation groups.
出处
《中国中西医结合急救杂志》
CAS
2008年第1期6-8,65,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然科学基金资助项目(30371826)
全军"十一五"课题基金资助项目(06MA251)
关键词
凉血活血方
中医药防治
肺损伤
放射性
prescription for cooling and promoting blood
prevention and treatment of traditional Chinese medicine
radioactive lung injury
