摘要
目的观察补肾对实验性变态反应性脑脊髓炎(EAE)大鼠生长相关蛋白-43(GAP-3)及微管相关蛋白-2(MAP-2)的影响,探讨其对轴突损伤的保护作用。方法应用髓鞘碱性蛋白(MBP)与完全福氏佐剂按1∶1(体积比)制成抗原并注射于大鼠双后足皮下,建立大鼠EAE模型。以醋酸泼尼松作对照,用苏木素-伊红染色(HE)观察各组大鼠造模后14 d、28 d脑和脊髓的组织病理变化,用轴突染色及免疫组织化学染色法观察脑和脊髓轴突损伤及再生情况。结果EAE模型组大鼠脑和脊髓组织中可见大量炎性细胞浸润,轴突存在明显损伤。脑和脊髓GAP-43及MAP-2表达显著降低,与正常对照组比较差异有统计学意义(P<0.05或0.01),分别用补肾方剂(左归丸和右归丸)干预后,大鼠炎症反应及轴突损伤较EAE模型组明显减轻,GAP-43及MAP-2表达明显高于模型组(P<0.05或0.01),以左归丸更为明显。结论补肾方剂能够上调GAP-43及MAP-2的表达,具有促进和增强轴突再生的作用。
Objective Multiple sclerosis（MS） is a common autoimmune neurodegenerative disease,which results in demyelination and axonal degeneration in brain and spinal cord.Recent studies have revealed that axonal loss is a major cause of permanent neurological and clinical disability.In the present study,the effect of on growth-associated protein-43（GAP-43） and microtubule-associated protein-2（MAP-2） expression in brain tissues of rat animal models of MS,namely experimental autoimmune encephalomyelitis（EAE） was observed.Methods The experimental rat postpeds were injected with antigen containing myelin basic protein（MBP） and Complete Freund＇s Adjuvant（CFA） with prednisone as control.The pathological changes in brain and spinal cord of rats were observed by hematoxylin-eosin（HE） staining and the injury of axon were observed by silver staining and immunohistochemistry method after the rat models were made 14 and 28 days late.Results It showed that there were abundant inflammatory cells infiltrating the cerebral and spinal cord tissues in EAE rats and sleeves formed around veins.Some of the nuclei of neuron were pyknotic The axons were damaged and the expressions of GAP-43 and MAP-2 in EAE model group were significantly less than those in normal control group（P〈0.05 or P〈0.01）.The inflammatory cells in foci comparatively decreased,sleeve-like changes were seldom seen,and the injuries of axons were reduced by treating with formulae or prednisone in EAE rats,Both in GAP-43 and MAP-2 expressions were increased significantly in groups treated with Zuo Gui and You Gui Pill（P〈0.05 or 0.01） compared with the untreated group,especially in rats treated with Zuo Gui Pill.Conclusion These results suggest that the can reduce the pathological reaction and increase the expressions of GAP-43 and MAP-2,thus promotes axon reconstruction and enhances its function.
出处
《首都医科大学学报》
CAS
2007年第6期748-752,共5页
Journal of Capital Medical University
基金
国家自然科学基金(30640069)
北京市自然科学基金(7062022)
北京市中医药科技项目(JJ2005-8)
北京市教育委员会科技发展计划项目(KM2007100025015)资助~~
关键词
补肾方剂
实验性变态反应性脑脊髓炎
轴突损伤
kidney-tonifying prescription,experimental allergic encephalomyelitis,axon injury
