摘要
目的:研究奈韦拉平(nevirapine,NVP)对HeLa细胞端粒酶活性和细胞周期的影响。方法:采用TRAP-PCR-ELISA方法检测HeLa细胞在奈韦拉平作用后端粒酶活性的变化,用流式细胞仪分析细胞周期的改变。结果:NVP作用后,HeLa细胞端粒酶活性明显抑制(P<0.05),并且HeLa细胞的G2/M期细胞含量明显增高(P<0.01)。结论:NVP对肿瘤细胞的端粒酶活性有抑制作用。
Objective： To study the effects of NVP on telomerase activity and cells phases in HeLa tumor cells. Methods： Telomerse activity was determined by TRAP-PCR-ELISA in untreated and treated HeLa tumor cells by NVP. Results： Telomerse activity of HeLa was significantly inhibited when treated with NVP（P 〈 0.05）,cell cycle of treated HeLa tumor cells were significantly increased in G2/M phase（P 〈 0.01）. Conclusion： NVP can effectively inhibit telom-erse activity of tumor cells.
出处
《天津医药》
CAS
北大核心
2007年第11期840-841,共2页
Tianjin Medical Journal
基金
武警总部级基金资助课题(项目编号:WY2003-02)
