摘要
采用平板划线分离法，从江西某铜矿酸性矿坑水中分离出一株极端嗜酸、又能耐受中度碱性条件的异养微生物，命名为HJM菌株。该菌株能在pH1．5～10．0的范围内生长。形态学以及18SrDNA和26SrDNAD1／D2区序列分析表明，HJM菌株属于P．guilliermondii这个种。金属抗性试验表明，该菌株对重金属铜离子的抗性可高达45mmol／L，因此，它的分离为研究极端环境中微生物的抗铜机制提供了材料。
By streak plate method, a strain of heterotrophic microorganism which was extremely acidophilic, alkalitolerant, named by HIM, was isolated from an acid mine drainage of a certain copper mineral in Jiangxi Province. This strain was able to grow under pH value of 1.5 - 10.0. Morphological characteristics and sequences analysis of 16S rDNA and 26S rDNA D1/D2 region revealed that it belonged to the species P. guilliermondii. Resistant experiment to metals showed that it could resist copper of 45mmol/L, so it was an important strain used to study the resistance mechanism of copper.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第5期921-925,共5页
Microbiology China
基金
国家自然科学基金创新群体基金(No50621063)
国家重大基础研究项目973基金(No2004CB619201)
