摘要
目的:测定止鼾胶囊中三七皂苷R1与人参皂苷Rg1、Rb1的含量。方法:采用HPLC,phenomenexC18柱(4.6mm×150mm,5μm),流动相:乙腈-水(20∶80)保持15min,15min后乙腈-水(40∶60)。流速:1.0mL.min-1,检测波长为203nm。结果:三七皂苷R1对照品在0.42～2.09μg线性关系良好,平均回收率为97.63%,RSD=1.16%(n=6);人参皂苷Rg1对照品在1.64～8.21μg线性关系良好,平均回收率为97.69%,RSD=1.49%(n=6);人参皂苷Rb1对照品在1.62～8.11μg线性关系良好,回收率为98.50%,RSD=1.70%(n=6)。结论:本实验三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量测定方法简单,结果稳定可靠,可作为止鼾胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的定量分析方法。
Objective： To quantitatively determine notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Zhihan Capsules. Methods： The HPLC procedure was used in the determination： the chromatographic column was phenomenex C18 （4.6mm × 150mm, 5μm）, the mobile phase was acetonitrile - water （20：80, 1 - 15min）, （40：60, after 15 min） . The flow velocity is 1.0mL· min ^-1, and the detection wavelength was 203 nm. Results： It was showed that notoginsenoside R1 had a good linear relationship when its concentration was 0. 42 -2. 091xg, and the sample recovery of the procedure was 97.63% （ RSD = 1.16%, n = 6 ） ; ginsenoside Rg1 had a good linear relationship when its concentration was 1.64 - 8.21 μg, and the sample recovery of the procedure was 97.69% （ RSD = 1.49% , n = 6） ; ginsenoside Rb1 had a good linear relationship when its concentration was 1.62 -8.11 μg, and the sample recovery of the procedure was 98.50% （ RSD = 1.70%, n = 6 ） . Conclusion ： The treatment of samples was simple and the results were accurate. The method can be used for extraction and determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Zhihan Capsules.
出处
《中国现代中药》
CAS
2007年第9期21-23,共3页
Modern Chinese Medicine
