摘要
2005年在云南省花卉公司种植的马蹄莲上发现有软腐症状,马蹄莲最初在受害部位出现水浸状坏死,病部很快扩大,病组织开始软化、变色,病斑边缘初有明显界限,随着病势的发展界限逐渐模糊不清,然后软腐.从病株上分离得到20株菌,菌株接种于马蹄莲上,发病症状与田间自然症状一致,并从回接病株上重新分离得到此病原细菌.经16S-23S rDNA转录间隔区PCR(ITS-PCR)分析、测序及BIOLOG生理生化测定,确定该病原菌为胡萝卜软腐果胶杆菌胡萝卜亚种(Pectobacterium carotovorasubsp.carotovora,p.c.c.)和菊果胶杆菌(Pectobacterium chrysanthemi,p.ch.)两种,而主要是Pectobacterium carotovorasubsp.carotovora,p.c.c..
Symptoms of soft rot were noticed on trumpet lily （Zantedschia） plants at some flower companies in Yunnan Province in 2005. The first symptoms of the disease on the plants were poor shoot growth and yellowing of the leaves. Then, small water-soaked lesions appeared on the tubers and stem bases. If conditions favoured disease development, the lesions rapidly enlarged, resulting in a soft and mushy decay. Twenty strains were isolated from diseased plants. Inoculation on the stem bases with the strains produced the same symptoms as those on the naturally infected stem bases. The pathogens were identified as Pectobacterium carotovora subsp, carotovor, p. c. c. and Pectobacterium chrysanthemi , P. ch. , according to morphological observation, biochemical and physiological identification, 16S-23S rRNA intergenic spacer sequences analysis and further pathogenicity determination.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第8期79-82,共4页
Journal of Southwest University(Natural Science Edition)
基金
云南省自然科学基金资助项目(2004C0007Z)
关键词
马蹄莲
细菌性软腐病
病原鉴定
Zantedeschia
Erwinia carotovora
patgogen identification
