摘要
目的:应用蛋白芯片技术,探讨不同类型慢性肝病患者血清中细胞因子的变化规律。方法:自行构建细胞因子芯片,并优化检测体系。选择健康人群(正常对照组,30例)及轻、中、重度慢性肝炎患者各30例(慢乙肝组),A、B、C级肝硬化患者各30例(肝硬化组),慢性重型肝炎30例(慢重肝组);检测各组患者血清中白细胞介素-2(IL-2)、γ-干扰素(IFN-γ)、IL-4、肿瘤坏死因子-α(TNF-α)、可溶性细胞间黏附分子-1(sICAM-1)、转化生长因子-β1(TGF-β1)、金属蛋白酶组织抑制剂-1(TIMP-1)7种细胞因子的水平。结果:慢乙肝组7种细胞因子与正常对照组比较差异均有显著性(P均<0.01),Th1类细胞因子IL-2、IFN-γ在慢乙肝轻度组中的水平基本正常,慢重肝组浓度最低(P<0.01)。Th2类细胞因子IL-4则以慢重肝组最高(P<0.01),其变化规律为轻度<A级<中度<重度=B级<C级<慢重肝,TNF-α变化规律为A级=轻度<中度=B级<C级<重度<慢重肝,TGF-β1为轻度<中度<重度<A级<慢重肝<B级<C级,sICAM-1则表现为轻度<A级<B级=中度<重度<C级=慢重肝,TIMP-1为轻度<中度<A级<重度<慢重肝=B级<C级(P均<0.01)。结论:慢性肝病患者血清细胞因子水平在一定程度上可反映肝脏炎症及纤维化程度,并且与慢性肝病的发生、发展、转归、预后密切相关。
Objective： To apply protein chip technology for studying the variation of serum cytokines in patients with chronic hepatopathy. Methods： Cytokine protein chip for ourselves were designed and detection system was optimized,the protein chip was used to analyze 7 cytokines in different kinds of persons,including healthy controls （n= 30） and following groups of patients., chronic hepatitis mild （CHMI）, chronic hepatitis moderate （CHMO）, chronic hepatitis severe （CHS）, liver cirrhosis A （LCA）, liver cirrhosis B（LCB）, liver cirrhosis C （LCC）, severe hepatitis（SH）,each group containing 30 cases. The levels of serum interleukin -2 （IL - 2）, interferon - 7 （IFN - T）, IL - 4, tumor necrosis factor -α （TNF -α）, soluble intercellular adhesion molecule - 1 （sICAM - 1）, transforming growth factor -β1 （TGF -β1）, tissue inhibitor of metalloproteinase - 1 （TIMP - 1） were detected. Results： The protein chip has better sensitivity and specificity. There were significant differences in 7 cytokines between patients and normal controls （all P〈 0.05）. Thl cytokines （IL- 2 and IFN -7） were normal in CHMI and significantly decreased in SH （P〈0. 01）. The level of Th2 cytokine （IL - 4） was highest in SH （P〈0. 01）, the regularity was CHMI〈LCA〈CHMO〈CHS=LCB〈 LCC〈SH. The regularity of TNF -α was LCA =CHMI〈CHMO = LCB〈LCC〈CHS〈SH〈 TGF -β1 showed CHMI 〈 CHMO 〈 CHS 〈 LCA 〈 SH 〈 LCB 〈 LCC ; sICAM - 1 had the rule of CHMI 〈 LCA 〈 LCB = CHMO〈CHS〈LCC=SH; the rule of TIMP- 1 was CHMI〈CHMO〈LCA〈CHS〈SH=LCB〈LCC （all P〈0.01）. Conclusion： The levels of serum cytokines in patients with chronic hepatopathy may reflect the degree of inflammation and fibrosis, and have intimate association with occurrence, development, turnover and prognosis of chronic hepatopathy.
出处
《中国中西医结合急救杂志》
CAS
2007年第5期267-270,F0002,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
广东省深圳市科技局资助项目(200304201)
关键词
蛋白芯片
慢性肝病
细胞因子
protein chip
chronic hepatopathy
cytokine
