摘要
目的:探索用蛋白芯片检测慢性肝病患者血清细胞因子的可能性。方法:制备了检测IL-2、IL-4和TNF-α细胞因子的蛋白芯片,并优化了检测条件。对34份临床标本进行检测,并与ELISA检测进行比较。结果:蛋白芯片检测结果与ELISA结果有很好的相关性,但蛋白芯片具有更好的灵敏性。结论:用蛋白芯片检测慢性肝病患者血清标志物是完全可能的。
Objective： To explore the possibility of detecting cytokines in the serum of patients with chronic liver disease by protein microarray.Methods： The protein microarray was made for detecting IL-2, IL-4 and TNF-α, and the test condition was optimized. Thirty four clinical specimens were tested with the protein microarray, and the results of protein microarray were compared with the results of ELISA. Results： The results of protein microarray is pertinent with the results of ELISA. But the sensi bility of protein microarray is better than that of ELISA.Conelusion： To detecte cytokines in the serum of patients with chronic liver disease by protein microarray has possibility.
出处
《中西医结合肝病杂志》
CAS
2006年第1期39-41,T0001,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
深圳市科技局课题(No:200304201)
关键词
蛋白芯片
细胞因子
慢性肝病
protein microarray
cytokines
chronic liver disease
