摘要
目的 建立枸杞子中甜菜碱含量的HPLC测定方法.方法 采用磺酸基团键合硅胶的阳离子交换剂（SCX）为填充剂;流动相为乙腈-0.05 mol·L^-1磷酸二氢钾-磷酸（15∶85∶0.18）;检测波长为192 nm.结果 甜菜碱浓度线性范围为0.40～2.40 μg;甜菜碱的平均回收率为98.99%,RSD为0.86%（n=6）.结论 该法灵敏度高,结果准确,可作为枸杞子中甜菜碱的定量测定方法.
Aim To establish a method for determination of betaine in lycium Chinese by HPLC.Methods The chromatographic column was shim-park Hypersil SCX（250 mm×4.6 mm,5.0 μm）.The mobile phase was acetonitrile-0.05 mol·L^-1potassium dihydrogen phosphate-phosphoric acid（15∶85∶0.18）,and UV detection was performed at 192 nm.Results The linear range was 0.40～2.40 μg,the average recover was 98.99%,and RSD was 0.86%（n=6）.Conclusion This method is accurate,reliable as well as specific and can be used to determine the content of betaine in lycium Chinese.
出处
《安徽医药》
CAS
2007年第8期703-704,共2页
Anhui Medical and Pharmaceutical Journal
