摘要
目的通过对沙门菌和志贺菌致泻血清型和耐药性研究了解菌型特征及耐药谱。方法收集和鉴定了115株沙门菌和644株志贺菌;以K-B法测试沙门菌和志贺菌对16种抗生素的耐药率,使用E-test定量法确认产超广谱β-内酰酶(ESBLs)菌株。结果肠炎沙门菌、鼠伤寒沙门菌和福氏志贺菌4c亚型(F4c)、宋内、福氏志贺菌2b亚型(F2b)分别是造成腹泻的优势血清型;比较2年中沙门菌的耐药率有上升趋势,耐四环素-萘啶酸-复方新诺明的R1型志贺菌比例高达79.91%,产ESBLs的分别有1株肠炎沙门菌、44株福氏志贺菌、11株宋内志贺菌。结论试管凝集效价是诊断不典型志贺菌型特异性抗原的定量依据,用E-test法判定的产ESBLs沙门和志贺菌符合复燃的新型肠道病原菌定义,志贺菌的耐药型谱对肠道门诊选用抗菌药物具有重要参考价值。
The serotype spectrum and antibiotics resistance characteritics of Salmonellae and Shigellae isolated from diarrhea patients in Shanghai were analyzed, in which the rates of drug-resistance to 16 antibiotics of these 115 isolates of almonellae and 644 isolates of Shigellae of which were well identified and serologically typed, were determined by K-B method, and the E-test method of quantitative confirmation was used to recognize that some of which could produce the extended spectrum-lactmases （ESBLs）. It was found that among these isolates, the dominant serotypes causing the development of diahhea consisted of the S. enteritidis, S. typhimurium and S. flexneri as well as the subtype 2b （F2b） of Sh. s. onnei and Sh. flexneri. In recent two years, the drug-fast rate of salmonellae showed a tendency to increase, in which the percentage of R1 type of shigellae that was multi-drug-resistant to terracycline, nalidixic acid and SMZ-TMP was high up to 79.91%. The isolates producing ESBLs consisted of one strain of S. enteriditis, 44 strains of S. flexneri and 11 strains of S. sonnei. From the above analysis, it is clear that the Salmonella and Shigella strains producing ESBLs as demonstrated by E-test method can define the newly developed infections. Meanwhile, the tube agglutination test titer seems to be the quantitative evidence to identify the specific antigens of atypical strains of S. flexneri, and the resistance spectrum of Shigellae has great referential value for diarrhea clinic to select antibiotics.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2007年第7期706-709,713,共5页
Chinese Journal of Zoonoses
