摘要
目的:通过体外、体内实验研究鸡血藤黄酮类组分的抗肿瘤作用。方法:采用MTT法,观察鸡血藤黄酮类组分体外对人肺癌(A549)和人大肠癌(HT-29)细胞系的生长抑制率;应用流式细胞术检测肿瘤细胞周期的改变。采用小鼠移植性Lewis肺癌模型,观察鸡血藤黄酮类组分体内的抑瘤效应及对荷瘤小鼠造血功能的影响。结果:鸡血藤黄酮类组分对人肺癌A549和人大肠癌HT-29细胞系有明显生长抑制作用,IC50分别为70.17±12.17μg.mL-1和126.81±43.00μg.mL-1。高剂量(0.6g.kg-1)灌胃时,对小鼠Lewis肺癌的抑制率为31.01%。该组分能促进红细胞生成,高剂量组红细胞计数比对照组增加40.52%。进一步研究发现,鸡血藤黄酮类组分能阻滞肺癌细胞系A549于S和G2/M期,阻滞肠癌细胞系HT-29于G2/M期。结论:鸡血藤黄酮类组分具有直接抗肿瘤作用,细胞周期阻滞是其药效作用机制之一。该组分无骨髓抑制作用,对红细胞生成有一定促进作用。
Objective： To observe the anti-tumor effect of the extract rich in flavonoids of Spatholobus suberctus Dunn in vitro and in vivo. Methods： In vitro, the inhibitory effects of the extract on proliferation of human lung carcinoma cell line A549 and human colorectal adenocarcinoma cell line HT-29 were measured by MTF assay. Cell cycle were analyzed using flow cytometry. The mouse model of Lewis lung carcinoma was used to investigate the effects of the extract on tumor growth and the hematopoietic system. Results： The studies demonstrated that the extract of flavonoids of Spatholobus suberctus Dunn inhibited proliferation of A549 and HT-29, and the ICso values on them were （70.17 ± 12.17） μg·mL^-1 and （126.81 ± 43.00） μg·mL^-1 respectively. The tumor inhibitory rate of high dose of extract on Lewis lung cancer was 31.01 %. The marrow-depression was not found and the peripheral blood red cell count increase to 40.82% in high dose group compared with the control. Furthermore, the extract arrests A549 cell in S and G2/M phase,but it induces HT-29 cell cycle arrest in G2/M phase only. Conclusions： The extract rich in flavonoids of Spatholobus suberctus Dunn exists direct anti-tumor effect and the mechanism of the anti-tumor activities may be the progression inhibition of cell cycle. Moreover,this extract can stimulate red blood cell production.
出处
《中国实验方剂学杂志》
CAS
2007年第2期51-54,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京市科技项目(H010910190119)
关键词
鸡血藤
黄酮
抗肿瘤
细胞周期
Spatholobus suberctus Dunn
Flavonoids
Anti-tumor
Cell cycle
