摘要
目的观察II型胶原诱导的胶原性关节炎(CIA)大鼠模型滑膜组织病理学特征及相关细胞因子表达水平的变化。方法60只健康雄性Wistar大鼠随机分为对照组(n=10)和模型组(n=50),模型组以牛源性II型胶原皮下注射诱导建立CIA模型,造模成功后随机选取10只大鼠进行病理观察并做免疫组化指标TNF-α、TGF-β检测,并与对照组进行比较。结果与对照组比较,模型组大鼠滑膜组织大量炎性细胞浸润、增生。对照组滑膜组织中TNF-α阳性滑膜细胞数呈低表达,且染色浅,模型组呈高阳性表达,深染色,2组比较差异有统计学意义(P<0.01);对照组滑膜组织中TGF-β表达较活跃,模型组则表达少而染色浅,2组比较差异有统计学意义(P<0.05)。结论细胞因子网络失衡与CIA大鼠的发病相关。
Objective To observe the pathological characters and expression of TNF-α and TGF-β of synovium in type 2 collagen-induced arthritis （CIA） rats.Methods 60 healthy male Wistar rats were randomly divided into model group （ n = 50） and control group （ n = 10） .The CIA model was made by injecting intradermally bovine type 2 collagen, After the CIA model was made successfully, 10 rats were selected to observe the pathological changes by HE staining and the expression of TNF-α and TGF-β was detected by immunohistochemical method. Results As compared with control group, there are a lot of inflammatory, cells in synovium tissues of rats in model group, and articular cartilage increased. The expression of TNF-α in control group was lower than that in model group and the staining color was lighter, on the contrary, The expression of TNF-α in model group was higher than that in control group and the staining color was deeper, there was a significant difference between two groups （ P 〈 0.01 ）The high expression of TNF-β in control group and low expression of TNF-β in model group were found, there was a significant difference between two groups （ P 〈 0.05）. Conclusion C ytokine network imbalance is closely correlated with pathogenesis of CIA rats.
出处
《疑难病杂志》
CAS
2007年第7期415-417,F0003,共4页
Chinese Journal of Difficult and Complicated Cases
关键词
关节炎
胶原性
细胞因子
病理特征
大鼠
Collageous arthritis
Cytokine
Pathological characteristics
Rats
