摘要
目的:通过观察骨量及生物力学指标,探讨中药壁虎提取物对影响骨组织构建正常结构骨质疏松的效应。方法:实验于2005-08/2006-07在解放军第四军医大学放射医学教研室完成。实验材料:健康8周龄雌性SD大鼠24只,体质量(220±15)g。壁虎提取物由解放军第四军医大学制备。实验分组:大鼠按体质量大小随机分为4组,即假手术组、模型组、预防组和治疗组,每组6只。实验干预:假手术组大鼠仅游离卵巢后关腹,其余各组大鼠均切除双侧卵巢。预防组大鼠术后6d开始灌胃用药,治疗组大鼠术后3个月开始灌胃用药,两组均按每日0.2g/kg体质量给予壁虎提取物1mL;假手术组和模型组大鼠术后6d给予等量注射用水灌胃。实验评估:术后6个月取各组大鼠骨组织,进行骨组织骨密度值、骨钙含量、最大应力、最大应变、弹性模量、骨皮质厚度等指标检测。结果:24只大鼠均进入结果分析。①骨密度和骨钙含量变化:假手术组与模型组相比各指标均有明显差异,表明骨质疏松动物模型制备是成功的。预防组和治疗组的全身骨密度值、腰椎骨密度值和骨钙含量均高于模型组大鼠(P<0.01)。②生物力学变化:预防组和治疗组的骨组织最大应力、最大应变及弹性模量高于模型组(P<0.01),但未能恢复到假手术组水平。③几何结构参数:预防组和治疗组的骨皮质厚度均较模型组增厚[(0.784±0.081),(0.686±0.077),(0.506±0.091)mm,P<0.01],两组的骨髓腔直径均较模型组降低[(1.993±0.149),(1.975±0.087),(2.120±0.098)mm,P<0.01和P<0.05]。结论:壁虎提取物可改善实验动物骨皮质厚度和髓腔径,增加骨量和骨强度,对骨组织构建影响因素骨质疏松有一定的防治作用。
AIM： To investigate the influence of the extracts of Chinese herb Gecko Swinhonis Gunther on osteoporosis through observing the bone content and biomechanical indexes, and discuss its effect on the construction of bone tissue. METHODS： The experiment was conducted in Staff Room of Radiation Medicine, Fourth Military Medical University of Chinese PLA from August 2005 to July 2006. Twenty-four healthy female SD rats of 8 weeks old and （220±15） g were randomly divided into control group, model group, prevention group and treatment group with 6 rats in each group. Except the control group, the others were all subjected to bilateral ovariectomy. 1 mL Gecko Swinhonis Gunther, which was provided by the Fourth Military Medical University of Chinese PLA, was intragastrfcally given to the rats of the prevention group 6 days after operation and the treatment group 3 months after operation at dose of 0.2 g/kg per day;. Rats in the control group and model group were given matching normal saline 6 days after operation. Six months later, all rats were executed to measure the bone mineral density, content of bone calcium, maximum stress, maximum strain, elastic modulus, and cortical thickness of bone. RESULTS： Twenty-four rats were involved in the result analysis. ①Compared with the model group, the bone mineral density and content of bone calcium of the control group were obviously changed, indicating that the animal model of osteoporosis was established successfully. The bone mineral density of the whole bone and lumbar vertebra, and bone calcium content in the prevention group and treatment group were obviously higher than those of the model group （P 〈 0.01）. ②The maximum stress, maximum strain, and elastic modulus of bone tissues in the prevention group and treatment group were higher than those of the model group （P 〈 0.01）, but did not recover to the level of the control group. ③The cortical thickness of bone of the prevention group and treatment group was thicker than that of the model group [（0.784±0.081）, （0.686±0.077）, （0.506±0.091） ram, P〈 0.01], but the diameters of marrow cavity of the two groups were all lower than that of the model group [（1.993±0.149）, （1.975±0.087）, （2.120±0.098） ram, P 〈 0.01, P 〈 0.05]. CONCLUSION： Gecko Swinhonis Gunther could increase the cortical thickness of bone and diameter of marrow cavity of rats, increase the bone intensity and content, and prevent and cure osteoporosis.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第23期4516-4519,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
