摘要
目的比较Vitek32鉴定系统和16S rRNA序列分析法鉴定凝固酶阴性葡萄球菌的灵敏度和特异性。方法选择2004年12月～2006年11月重症监护病房分离的凝固酶阴性葡萄球菌70株，常规细菌培养后，用Vitek32鉴定系统和16S rRNA基因测序进行分类，以API Staph系统为参照，比较2种鉴定方法的优势和不足。结果根据Vitek32鉴定系统判断标准，将鉴定结果分为3组：Ⅰ组（25株）：“不可接受”，％id〈80；Ⅱ组（35株）：“可接受的”，％id80～90；Ⅲ组（10株）：“好的”，％id90～98，标准菌株为金黄色葡萄球菌（ATCC29213），分别进行16S rRNA基因测序。16S rRNA基因分析法全部鉴定到种，2种方法鉴定符合率分别为Ⅰ组64％、Ⅱ组74．3％、Ⅲ组100％，总体鉴定符合率74．4％。结论Vitek32在进行凝固酶阴性葡萄球菌鉴定时有局限性，16SrRNA序列分析可以弥补鉴定上的不足，在发展难培养和不能培养微生物的鉴定中将发挥重要作用。
Objective To compare the sensitivity and specificity in identifying coagulase negative staphylococcus （CNS） between Vitek32 microbic system and 16S rRNA sequence analysis. Methods 70 strains of CNS isolated from Intensive Care Unit（ICU） the time period from during December 2004 through November 2006 were chosen to perform convention becilliculture, then they were classified as Vitek32 microbic system and 16S rRNA sequence analysis, and the superiority and shortage of these two identification methods were compared. Results The identifying results were divided into three groups according to Vitek32 system standard. Group Ⅰ, ‘unacceptable group＇ % id 〈 80, had 25 strains; Group Ⅱ , ‘acceptable group＇ % id 80 ～ 90, had 35 strains ; and Group Ⅲ, ‘Good group＇ % id 90 ～ 98, had 10 strains. The standard strain was the Staphylococcus aureus（ ATCC29213 ）. All these strains were carried out 16S rRNA gene sequencing respectively. The coincidence rates of group Ⅰ , group Ⅱ and group Ⅲ using these two methods were 64%, 74.3% and 100%, respectively. The overall coincidence rate was 74.4%. Conclusion 16S rRNA sequence analysis could make up the limitation of Vitek32 system in identifying CNS. It will play an important role in identifying the microorganisms that are difficult to culture or those could not be cultured.
出处
《首都医科大学学报》
CAS
2007年第2期150-153,共4页
Journal of Capital Medical University
基金
国家自然科学基金(30672006)资助项目
关键词
全自动微生物分析仪
序列分析
凝固酶阴性葡萄球菌
the automatic microorganism analyzer
sequence analysis
coagulase negative staphylococcus（CNS）
