摘要
目的探讨PTEN基因变异在原发性肝细胞癌(HCC)发生发展中的作用。方法应用半定量逆转录聚合酶链反应(RT-PCR)技术,对30例肝癌组织、30例癌旁组织和16例正常肝组织细胞中PTEN基因的表达情况进行了检测和分析。结果肝癌组织中PTEN基因表达水平明显低于癌旁组织和正常肝组织(F=6.785,q=4.269、4.549,P<0.05)。低分化肝癌组织中PTEN基因表达水平显著低于高、中分化肝癌组织(t=3.266,P<0.01)。结论PTEN基因失活与肝癌发生密切相关;PTEN基因表达水平与HCC恶性程度有明显相关性,提示其变异可以作为病人预后判断的指标。
Objective To study the role of PTEN variation in the pathogenesis of hepatocellular carcinoma （HCC）. Methods Semi-quantitive reverse transcriptase polymerase chain reaction （RT-PCR） was adopted to detect the expression of PTEN gene in 30 HCC and their adjacent tissues as well as 16 normal liver tissues served as controls. Results The expression level of PTEN gene in the HCC tissues was significantly lower than that in the adjacent and normal liver tissues （F= 6. 785;q= 4. 269,4. 549;P〈0, 05）. In lowly-differentiated HCC tissue, the expression of PTEN was much lower than that in the highly- and moderately-differentiated HCC tissues （t=3. 266,P〈0.01）. Conclusion The inactivation of PTEN is closely associated with the pathogenesis of hepatocellular carcinoma, and the expression of PTEN is highly related with the malignancy of the cancer, which suggests that the alternation of PTEN can serve as a prognostic predictor for this disease.
出处
《青岛大学医学院学报》
CAS
2007年第2期153-155,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
基因
PTEN
RNA
信使
肝细胞癌
逆转录聚合酶链反应
gene, PTEN
RNA, messenger
carcinoma, liver cell
reverse transcriptase polymerase chain reaction
