摘要
目的:探讨α-干扰素(IFN-α)和γ-干扰素IFN-γ对淋巴管内皮细胞增殖和游走的影响及作用机制。方法:内皮细胞取自猪的胸导管。应用Hoechst和Caspase染色法检测IFN-α和IFN-γ诱导的细胞凋亡;采用划线法和MTT法观察IFN-α和IFN-γ对淋巴管内皮细胞增殖和游走的影响;活兔后肢切断淋巴管并分别注射IFN-α和生理盐水,观察淋巴管的再生情况。结果:经IFN-α和IFN-γ处理后,淋巴管内皮细胞其核周围有凋亡小体;Caspase染色阳性表达;IFN-α和IFN-γ对淋巴管内皮细胞的增殖和游走有明显的抑制作用;手术切断兔后肢淋巴管后16d,IFN-α侧仍有大量染料渗漏。结论:IFN-α和IFN-γ具有促进细胞凋亡的作用。IFN-α和IFN-γ对淋巴管内皮细胞的增殖和游走有明显的抑制作用。
Objective： To study the effect and mechanism of IFN-α and IFN-γ on proliferation and migration of lymphatic endothelial cells（LEC）. Methods： LECs were taken from the pig thoracic duct. Hoechst and Caspase staining was applied to examine apoptosis of LECs. Method of scraping line and MTT were used to detect IFN-α and IFN-γ on proliferation and migration of LECs. Lymphatics of two posterior limbs in rabbits were cut and IFN-α and 0.9% NaCl solution were injected around the lymphatic vessels of each limb respectively to observe healing of the lymphatics. Results： After the treatment with IFN-α and IFN-γ, the apoptotic body was found surrounding the nucleus of LECs. Caspase staining proved the positive cells of apoptosis. IFN-α and IFN-γ could evidently inhibit proliferation and migration of LECs. IFN-α experiment in vivo ： 16 days after the operation, the lymphatic vessels which had been injected with IFN- α solution had a serious leak. Conclusion： IFN-α and IFN-γ can promote apoptosis of LECs and evidently inhibit the proliferation and migration of LECs.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2007年第1期16-21,共6页
Chinese Journal of Anatomy
基金
青岛市科技局基金(02-1-kj-yj-49)
关键词
干扰素Α
干扰素Γ
淋巴管
内皮细胞
细胞增殖
细胞游走抑制
interferon-α
interferon-γ
lymphatic vessels
endothelial cells
cell proliferation
cell migration inhibition
