摘要
黄酮类化合物因具有多种生物活性而成为目前天然产物研究的热点。本研究通过三种检测方法的比较,确定了聚酰胺-UV法为蜂花粉总黄酮的测定方法,其检测波长为360nm,以芦丁为标准品,在0.005～0.025mg/ml范围内,其浓度与吸光度呈良好的线性关系,相关系数R2为0.9998,平均回收率分别为95.53%,稳定性、精密度和重现性均理想,其RSD分别为2.124%(n=3)、0.488%(n=3)、0.875%(n=5)。该法检测油菜蜂花粉中总黄酮含量为1.54%。
Flavonoids are widely studied in natural products because of their multifarious bioactivities to human body. The paper compared tree methods of determination and confirmed a method for quantitative determination of total flavonoids in beepollen by ployamide-UV spectrophotometry. The standard sample was rutin. The optimum determination wavelength was 360nm. The results showed that a good linear relationship （R2=0.9998） between OD and the concentration of analyte is obtained,within the range of 0.005~0.025mg/ml. The average recovery rate is 95.53%. The stability, precision and repetition are very good, with RSD 2.124%（n=3）, 0.488%（n=3）, 0.875%（n=5） respectively. The content of the total flavonoids in rape pollen is 1.54% by ployamide-UV spectrophotometry.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第1期262-265,共4页
Food Science
关键词
蜂花粉
总黄酮
聚酰胺-UV法
比色法
双波长比色法
bee-pollen
total flavonoids
ployamide-UV
colorimetry
dual wavelengh colorimetry
