摘要
黄酮类成分因其具有多样生物活性成为目前天然产物研究的热点。本实验探讨了聚酰胺-UV法作为蜂花粉总黄酮测定方法的可行性。结果表明以芦丁为标准品,以360nm为检测波长,用聚酰胺-UV法检测蜂花粉总黄酮在0.005-0.025mg/mL范围内,浓度与吸光度呈良好的线性关系,相关系数R2为0.9998,回收率、稳定性、精密度和重现性均理想,其RSD分别为2.48%(n=3),2.12%(n=3),0.49%(n=3)、0.88%(n=5)。该法检测油菜蜂花粉中总黄酮含量为1.54%。
Flavonoid is widely researched in nature product exploitation because of its specific activity to human body. The present studies developed a method for quantitative determination of the total content of beepoUen flavonoids via ployamide-UV spectrophotometry. The contrast sample was rutin for ployamide-UV spectrophotometry. The optimum determination wavelength was 360nm. The result showed that a good linearity relationship （correlation coefficient： 0.9998） between the degree of absorbance and the concentration of analyte was obtained, within the range of 0.0050.025mg/ml. The average recovery rates were 95.5%, the measurement precision was high with its RSD 0.488%（n=3）.
出处
《中国蜂业》
2006年第8期8-10,共3页
Apiculture of China
关键词
蜂花粉
总黄酮
聚酰胺-UV法
beepollen
total flavonoids
ployamide-UV spectrophotometry
