摘要
目的探讨鱼腥草阻断甲1型流感病毒诱导MDCK(Madin-Darby canine kidney MDCK)细胞程序化死亡(凋亡)的效果。方法采用姬姆萨染色光镜下观察细胞形态变化,annexinV/PI染色流式细胞技术测定。结果1病毒感染组MDCK细胞凋亡百分率显著高于其它组(P<0.01);2试验组分别与药物对照和细胞对照组比较无差异(P>0.05)。结论该研究提示甲1型流感病毒可诱导MDCK细胞凋亡,而鱼腥草能阻断甲1型流感病毒诱导MDCK细胞凋亡。
Objective To investigate inhibitory effects of Yu Xing Cao on the apoptosis induced by influenza virus A： in MDCK（Midin darby canine Kidaney） cells .Methods The morphological changes of apoptotic cells under light microscopy. Influenza virus A1 induced apoptotic cell percentage was calculated through two methods： annexinV/PI and Giemsa stalning.Rcsults The percentage of MDCK cells apoptosis of virus controt was significantly higher than that of Yu Xing Cao and virus combined treated group cell control and drug control （P〈0.01） .While no significance difference was shown among the two latter（P〉0.05） all in the Giemsa, annexinV/PI. Conclusion This study suggest that Yu Xing Cao can against the apaptosis indluced by in fluenut virus A1.
出处
《江西医药》
CAS
2006年第12期960-961,共2页
Jiangxi Medical Journal
关键词
流感病毒
鱼腥草
流式细胞术
apoptosis
influenza virus A1
Yu Xing Cao
tlow cytometry
