摘要
目的探讨心肌型脂肪酸结合蛋白(H-FABP)在急性心肌梗死(AMI)早期区分AMI患者与正常人的能力。方法人为纳入正常人、AMI确诊病人比例2:1的小样本人群,采用双抗体夹心ELISA法对40例正常人与19例AMI患者血清H-FABP浓度进行定量,绘制H-FABP在该人群中用于诊断AMI的受试者特征曲线(ROC曲线)并比较曲线下面积(AUC)与0.5的大小。0.5被认为是确定一项指标是否具有任何诊断意义的AUC临界值。结果H-FABP浓度定量正常人组为1.29±0.64 ng/mL AMI组为24.45±32.40 ng/mL;依此绘制出的ROC曲线其AUC为0.978(95%CI∶0.948-1.000),与0.5比较差异具有统计学意义。结论H-FABP具备在心梗早期区分AMI与正常人的能力,H-FABP定量检测用于AMI早期诊断是具备其可行性的。
Objective To estimate the ability of Heart-type fatty acid-binding protein for distinguish of AMI patients and healthy individuals in the early phase of myocardial infarction.Methods A manual population was built by ruling in healthy individuals and AMI patients with a ratio of 2：1. Serura H-FABP concentrations were measured in the 40 healthy individuals and 19 AMI patients by sandwich ELISA,then the value of the two groups was compared; ROC curve for H-FABP in diagocsing AMI patients in the manual population was then built,the AUG was calculated and compared with the value of 0.5. The latter was defined as the critical value to distinguish whether a new marker had the diagnosis ability or not. Results the concentrations of H-FABP in AMI patients and healthy individuals were quantification as 1.29 ± 0.64 ng/mL and 24.45 ± 32.40 ng/mL respectively; AUC d the ROC was calculated and the result is 0.978 （95 % C I：0.948 - 1.000）, which was conspicuously larger than 0.5. Conclusion H-FABP has the ability for distinguish AMI patients from healthy individuals in the early phase of myocardial infraction and may be used as a myocaatial indicator in the early phase of MI.
出处
《中国实验诊断学》
2007年第1期63-65,共3页
Chinese Journal of Laboratory Diagnosis
基金
广东省医学科研基金资助(项目编号:A2006404)
关键词
脂肪酸类
心肌梗死
生化标志物
fatty acids
myocardial infarction
biochemical marker
