摘要
利用柠檬酸钠还原氯金酸,形成胶体金颗粒,选择直径20 nm的胶体金颗粒标记单克隆抗体4E8,用兔抗鼠二抗喷洒在硝酸纤维素膜上作为对照线,用另一株单抗5E6作为检测线,制备成胶体金试纸条。用试纸条检测120份泄殖腔样品,结果与病毒增殖后HA法测得的结果符合率为95.8%,HA效价在1∶2以上,试纸条均能检测为阳性,与其他病毒的阳性血清没有交叉反应,且室温下可有效保存6个月。试纸条应用方便、快速,适合临床推广以及流行病学调查。
To develop a rapid, simple and high specific gold immunoehromatography assay （GICA） for the diagnosis of N DV. Tri - sodium citrate with aqueous gold chloride were wanned up and mixed to make gold sol. Monoelonal antibedy4E8 was labeled by colloidal gold whose diameter is 20 nm. The antibody of rabbit against mouse was fixed on nitrocellulose membrane （NCM） that was the control line;the test line was another monoelonal antibedySE6. 120 samples were detected for NDV by GICA and HA. The correspondence rate between the colloidal gold strip and HA was 95.8 %. If the HA titer was above 1： 2, the GICA result would be positive. There were no crossing reflections with other virus and the strips could store six months. Colloidal gold strip was a rapid method to develop in clinic practice and could be very useful in surveillance of antibody to Newcastle Disease virus.
出处
《西南农业学报》
CSCD
2006年第6期1162-1165,共4页
Southwest China Journal of Agricultural Sciences
