摘要
基于免疫竞争胶体金免疫层析原理,研制了检测食用肉中呋喃妥因代谢物1-氨基乙内酰脲(AHD)的免疫试纸条。用柠檬酸钠还原法制备胶体金颗粒,标记抗1-氨基乙内酰脲的衍生物(CPAHD)的单克隆抗体并喷于玻璃纤维上,CPAHD-BSA(Bovine serum albumin)抗原和羊抗鼠IgG分别结合于硝酸纤维膜上,依次将样品垫、胶体金垫、硝酸纤维素膜和吸水纸组装切割成AHD胶体金免疫层析快速检测试纸条。在5 min内肉眼观察结果,该试纸条对AHD的最低检测限为2.33μg/L,除与呋喃妥因有弱交叉反应外,与其他同类物均无交叉反应,用该试纸条和ELISA(Enzyme linked immunosorbent assay)检测猪肉中添加的AHD,结果呈现很好的相关性。该方法灵敏度高,简便快速,无需特殊仪器设备,可作为呋喃妥因残留批量检测的筛选方法。
A method of colloidal gold immunochromatographic assay for the determination of 1-aminohydantoin（AHD） in pork was established based on competition principle.Colloidal gold marked with monoclonal antibody against AHD′s derivates（CPAHD）was sprayed onto the glass fiber.CPAHD was conjugated bovine serum albumin（BSA） and goat anti-mouse immunoglobulins were jet-positioned onto anitrocellulose membrane.Sample pad,gold jet-pad,nitrocellulose membrane and absorbent paper were assembled and cut into detecting strips,respectively.The whole assay could be finished in 5 min.The limit of detection for the strips,which could be read by naked eyes,was 2.33 μg/L.No cross-reaction with other analogs of AHD occurred except for nitrofurantoin.A good consistency was obtained between results of AHD determination by this method and the enzyme linked immunosorbent assay（ELISA） method.The proposed method was rapid,easy,sensitive,and could be widely used to detect the nitrofurantoin residues,with no need for special equipment.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第7期680-685,共6页
Journal of Instrumental Analysis
基金
广东省部"产学研"资助项目(2009B090300452)
