摘要
利用组织培养对内蒙古特色资源的河套苹果梨进行遗传保护、品质改良以及生物技术操作具有重要的理论意义和实践价值。试验利用NAA、2，4-D和6-BA对苹果梨叶片进行组织脱分化研究。结果表明，0-0．2mg／L的NAA与0．1mg／L的6-BA组合可有效促进叶片组织脱分化，形成愈伤组织或直接诱导叶芽的萌发。NAA与2，4-D组合未表现出有明显促进河套苹果梨叶片组织脱分化的作用。在叶片组织脱分化过程中，外植体的染菌率与激素水平存在一定的线性关系。
In order to accomplish the research project of Hetao apple-pear＇s tissue culture, this study conducted a primary study of tissue dedifferentiation of Hetao apple-pear under the effects of the three plant hormones NAA, 2,4-D and 6-BA. The results indicated that the effects of combination of NAA（0- 0.2 mg/L） and 6-BA（0.1 mg/L） can effectively dedifferentiate the vegetative tissue （buds） of Hetao apple-pear, and stimulated callus formation or directed growth of leave buds. The combinations of NAA and 2,4-D had potential effects on tissue dedifferentiation of Hetao apple-pear. Meanwhile, it was found that the intensity of microorganism contamination of explants during tissue culturing were correlated to the concentration of plant hormone.
出处
《华北农学报》
CSCD
北大核心
2006年第6期130-134,共5页
Acta Agriculturae Boreali-Sinica
基金
内蒙古自然科学基金项目(20010903-11)和(200408020321)资助
关键词
河套苹果梨
植物激素
组织脱分化
因素互作
Hetao apple-pear
Plant hormone
Tissue dedifferentiation
Combined effects
