摘要
目的比较心肌型脂肪酸结合蛋白(H-FABP)、肌钙蛋白(C-TnI)、肌酸磷酸激酶同功酶(CK-MB)在早期心肌梗死诊断中的作用.方法将20只新西兰白兔随机分为手术组(n=12)和假手术组(n=8),手术组开胸结扎冠状动脉左前降支,假手术组开胸穿线不结扎冠状动脉,分别抽取手术前和术后1、2、3、4、6、8、10、24 h血液,均采用酶联免疫吸附试验法分别测定H-FABP、C-TnI、CK-MB浓度.结果两组间比较分别于术后1 h H-FABP差异有显著性(P<0.05),术后2 h cTnI差异有显著性(P<0.05),术后4 h CK-MB差异有显著性(P<0.05).H-FABP平均升高时间为1.4 h,比C-TnI(2.6 h)和CK-MB(4.0 h)提前(P<0.05).结论H-FABP在急性心肌梗死中比C-TnI和CK-MB提前升高,是一种有价值的急性心肌梗死早期诊断生化标志物.
Objective To evaluate the diagnostic value of H - FABP, cTnI and CK - MB for early detection of acute myocardial infarction （AMI） . Methods 20 New -Zealand rabbits were randomly divided into 2 groups： surgery group （MI group , n = 12） and sham surgery group （control group, n =8） . MI group was opened the chests and the anterior descending branches of the left coronary arteries （LAD） were ligated; in sham surgery group the sutures were passed under the LADs without ligation. Blood sampled before surgery and 1, 2, 3, 4, 6, 8, 10 and 24 h after surgery. H - FABP, cTnI and CK - MB were measured by ELISA respectively at the same time. Results The plasma concentrations of H - FABP 1 hour after surgery showed statistical difference between MI group and sham surgery group （P 〈 0. 05 ） ; The plasma concentrations of eTnI 2 hours after surgery showed statistical difference between MI group and sham surgery group （ P 〈 0. 05 ） ; The plasma concentrations of CK - MB 4 hours after surgery showed statistical difference between MI group and sham surgery group （P 〈 0. 05 ） . The mean increased time of H - FABP was I. 4 h, which was faster than cTnI （2.6h） and CK-MB （4.0h） （P〈0.05） . Conclusions H-FABP increased before cTnI and CK-MB in AMI. was a valuable biochemical marker for the early detection of AMI.
出处
《昆明医学院学报》
2006年第6期65-69,共5页
Journal of Kunming Medical College
基金
云南省教育厅基金资助(5J0443C)
昆明医学院基金资助(12-13)
昆明医学院第一附属医院博士启动基金资助(20904-107)
