摘要
目的:观察中药木犀草素对哮喘小鼠气道重塑的抑制作用并探讨其可能机制。方法:实验于2006-01/04在南京医科大学呼吸病研究室完成。SPF级雄性BALB/c小鼠32只,体质量18～22g,随机数字表法分为正常对照组、哮喘模型组、地塞米松组、木犀草素组,每组8只。卵蛋白致敏反复雾化吸入共8周,建立慢性哮喘模型,每次雾化前30min,正常对照组、哮喘模型组生理盐水灌胃,地塞米松组、木犀草素组分别使用地塞米松及木犀草素进行干预。观察支气管肺泡灌洗液中白细胞介素5和γ-干扰素水平的变化。对肺组织切片行苏木精-伊红染色,借助图像分析软件测量基底膜周径、平滑肌面积、管壁面积,计算出支气管管壁厚度和平滑肌厚度。结果:纳入小鼠32只,均进入结果分析。①经过反复抗原激发,哮喘模型组肺泡灌洗液中白细胞介素5水平明显高于正常对照组、地塞米松组和木犀草素组犤(50.7±2.9),(16.1±1.2),(26.8±1.5),(25.9±2.6)ng/L,P<0.05犦。②γ-干扰素水平明显低于正常对照组、地塞米松组和木犀草素组犤(38.1±3.3),(64.2±3.5),(58.2±4.2),(54.3±2.4)ng/L,P<0.05犦。③哮喘模型组支气管管壁厚度和平滑肌厚度明显高于正常对照组、地塞米松组和木犀草素组犤管壁厚度:(17.1±1.6),(12.7±1.1),(14.3±1.1),(14.0±1.2)μm;平滑肌厚度:(6.0±0.5),(4.5±0.4),(5.0±0.5),(5.2±0.6)μm,P<0.05犦。结论:木犀草素可抑制气道壁的增厚和平滑肌增殖,减轻气道重塑。
AIM： To observe the inhibitory effect of luteolin on the airway remodeling in asthmatic mice, and explore the related mechanism. METHODS： The experiment was conducted in the Research Laboratory of Respiratory Disease, Nanjing Medical University from January to April 2006. Thirty-two male SPF grade BALB/c mice of 18-22 g were randomly divided into control group, asthma group, dexamethasone （DXM） treated group and luteolin treated group with 8 mice in each group. The mice were sensitized and repeatedly challenged with lbumin for 8 weeks to establish the models of chronic asthma, and 30 minutes before atomization, the control group and asthma group were infused with normal saline, the DXM group was infused with DXM and the luteolin group was infused with DXM and luteolin, respectively. The changes of interleukin-5 and interferon gamma （IFN-γ） levels in the bronchoalveolar lavage fluid were observed. The lung tissue sections underwent HE staining to measure the bronchial basement membrane perimeter （Pbm）, smooth muscle area （WAm） and bronchial wall area （WAt） by image analysis software, then the airway smooth muscle thickness （WAm/Pbm） and airway wall thickness （WAt/ Pbm） were calculated. RESULTS： All the 32 mice were involved in the result analysis. ①After repeated challenges, the interleukin-5 level in bronchoalveolar lavage fluid of the asthma group were significantly higher than the control group, DXM group and luteolin group [（50.7±2.9）, （16.1±1.2）, （26.8±1.5）, （25.9±2.6） ng/L, P 〈 0.05]. ②The IFN-γlevel of the asthma group were markedly lower than the control group, DXM group and luteolin group [（38.1±3.3）, （64.2±3.5）, （58.2±4.2）, （54.3±2.4） ng/L, P 〈 0.05]. ③The airway wall thickness and airway smooth muscle thickness of the asthma group were obviously higher than the control group, DXM group and luteolin group [WAt/Pbm： （17.1±1.6）, （12.7±1.1）, （14.3±1.1）, （14.0±1.2）μm; WAm/Pbm： （6.0±0.5）, （4.5±0.4）, （5.0±0.5）, （5.2±0.6）μ, P 〈 0.05]. CONCLUSION： Luteolin might inhibit the airway wall thickening and airway smooth muscle proliferation to lessen the airway remodeling.
出处
《中国临床康复》
CSCD
北大核心
2006年第47期82-84,共3页
Chinese Journal of Clinical Rehabilitation
