摘要
背景:微量元素与人体健康的关系日益受到重视,许多微量元素是机体必不可少的。微量元素的缺乏与心脑血管疾病的发生有着不容忽视的密切关系,而中药的药效则决定于它所含微量元素的种类和含量。目的:测定10种治疗脑血管疾病的中成药中钙(Ca)、镁(Mg)、铜(Cu)、铬(Cr)、锌(Zn)和铁(Fe)6种金属元素的含量。单位:吉林医药学院医学实验中心。材料:实验于2003-03/2003-05在吉林化工学院进行。选择脑塞通、强力天麻杜仲丸、抗栓丸、通脉宁心冲剂、溶栓胶囊、步长脑心通、大活络丹、中风回春丸、消栓通络胶囊、华佗再造10种治疗心脑血管疾病的中成药,均为市售。浓HNO3,优级纯,北京化工厂生产;HClO4,优级纯,天津市东方化工厂生产。方法:将样品丸剂取10丸,片剂取10片,分别粉碎混匀。每一样品平行取2份,每份1.00g,分别置于10个50mL锥形瓶中,加入浓HNO310mL封口过夜。次日在锥形瓶中分别加入浓HNO310mL,HClO45mL,于电热板上缓慢加热,至剩余2mL左右时再分别加入浓HNO320mL,HClO45mL,继续消化至溶液呈无色透明近干为止,冷却后分别将药品转移至25mL容量瓶中,用3%HNO3定容。采用原子吸收分光光度法分别测定10种中成药中Ca、Mg、Cu、Cr、Zn、Fe6种金属元素的含量。主要观察指标:10种中成药中Ca、Mg、Cu、Cr、Zn、Fe6种金属元素的含量。结果:10种药物中金属元素含量:溶栓胶囊中Mg含量较多,为(89.2016±0.8554)mg/g;大活络丹中Ca含量最多,为(14.3125±0.5070)mg/g;抗栓丸中Zn、Fe含量丰富,分别为(60.4875±1.6398)mg/g和(980.0812±15.3959)mg/g;而消栓通络胶囊中含Cr较多,为(9.8812±0.7344)mg/g。结论:10种药物中的金属元素含量都比较丰富,但存在一定差异。显示结果为探讨中成药金属元素含量与治疗脑血管疾病疗效的关系提供了一定依据。
BACKGROUND： The relationship of trace element and human body health is increasingly paid more attention. Many trace elements are indispensable for organism. The lack of trace element is closely related to the occurrence of eardio-cerebrovaseular disease, while the medieative effect of traditional Chinese medicine depends on the sorts and level of the contained trace elements.
OBJECTIVE： To determine the level of Ca, Mg, Cu, Cr, Zn and Fe in 10 traditional Chinese medicine patent prescriptions for the treatment of cerebrovascular disease.
SETTING： Medical Experimental Center of Jilin Medical College.
MATERIALS： This experiment was carried out in the Jilin Institute of Chemical Technology during March to May 2003. Ten traditional Chinese medicine patent prescriptions Nansaitong, Qiangli Tianma Duzhong Wan, Kangshuan Wan, Tongmai Ningxin Chongji, Rongshuan Jiaonang, Bachang Naoxintong, Dahuoluodan, Zhongfeng Huichun Wan, Xiaoshuan Tongluo Jiaonang and Huatuo Zaizao Wan, purchased from market were selected for the treatment of cardio-cerebrovaseular disease in this study. Strong HNO3 and grade-guaranteed reagent were made in Beijing Chemical Co.Ltd; HClO4 and another kind of grade-guaranteed reagent were made in Tianjin Dongfang Chemical Co.Ltd.
METHODS： Ten pills and 10 tablets were chosen and crushed evenly respectively. Two portions were equally chosen from each sample, 1.00 g each portion, and placed in ten 50 mL conical flask respectively. Then, strong HNO3 of 10 mL was used to envelop the conical flask, overnight. On the next day, strong HNO3 of 10 mL and HClO4 of 5 mL were respectively added into each flask, then which was slowly heated on the electric hot plate. When about 2 mL liquid was left in the conical flask, another 20 mL strong HNO3 and 5 mL HClO4 were respectively added to further digest till the solution was achromatic and transparent, and flask was nearly dried. After cooled, the samples were transferred to a 25 mL volumetric flask and metered with 3% HNO3. The level of Ca, Mg, Cu, Cr, Zn and Fe 6 metallic elements in 10 traditional Chinese medicine patent prescriptions was measured respectively with atomic absorption spectrophotometry.
MAIN OUTCOME MEASURES： The level of Ca, Mg, Cu, Cr, Zn and Fe 6 metallic elements in 10 traditional Chinese medicine patent prescriptions.
RESULTS： Among the levels of metallic elements in 10 traditional Chinese medicine patent prescriptions, the level of Mg was found the most in Rongshuan Jiaonang [（89.201 6±0.855 4） mg/g]; The level of Ca the most in Dahuoluodan [（14.312 5±0.507 0） mg/g]; Zn and Fe the most abundant in Kangshuan Wan [（60.487 5±1.639 8）mg/g and（980.081 2±15.395 9） mg/g respectively]; while the level of Cr very more in the Xiaoshuan Tongluo Jiaonang [（9.881 2±0.734 4） mg/g].
CONCLUSION： Six metallic elements are all abundant in 10 traditional Chinese medicine patent prescriptions, but significant differences exist. Experimental results provide some evidences for investigating the relationship of the levels of metallic elements in 10 traditional Chinese medicine patent prescriptions and the therapeutic effect on treating cerebrovascular disease.
出处
《中国临床康复》
CSCD
北大核心
2006年第43期225-228,共4页
Chinese Journal of Clinical Rehabilitation
