摘要
建立了同时检测海产品中土霉素、四环素、金霉素、氯霉素残留的高效液相色谱分析方法，以醋酸铵-醋酸／水缓冲液和乙腈作为流动相，通过二元梯度洗脱，使四种抗生素获得了较好的分离，以基线噪音的3倍计算，土霉素、四环素、金霉素、氯霉素的最低检出浓度分别为32、15、23、19vg／kg，在添加浓度范围内，四种抗生素的加标回收率为76％～92％，相对标准偏差为0．8％～4．5％，该方法快速、灵敏、准确，可靠性高，可用于海产品中抗生素残留的色谱分析。
A method for simultaneous determination of oxytetracycline, tetracycline, chlortetracycline and choramphenicol in seafood was successfully developed using high performance liquid chromatography with ammonium acetate/water and acetonitrile as the mobile phase. The results showed that the four antibiotics were well separated under the optimum conditions, with the limits of detection being 32 μg/kg for oxytetracycline, 15 μg/ kg for tetracycline, 23 μg/kg for chlortetracycline, and 19 μg/kg for choramphenicol. The recovery of standard additions was 76 %- 92 % and the relative standard tire, accurate and reliable and can be applied in the deviation was 0.8 % - 4.5 %. The method is rapid, sensianalysis of antibiotic residues in seafood
出处
《北京化工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2006年第5期90-93,共4页
Journal of Beijing University of Chemical Technology(Natural Science Edition)
基金
国家'十五'重点科技攻关项目(2002BA906A28-1B)
关键词
抗生素
液相色谱
分离
检测
海产品
antibiotic
liquid chromatography
separation
determination
seafood
