摘要
目的研究人博卡病毒检测方法与小儿感染的临床特征,以提高对小儿人博卡病毒感染的诊治技术。方法采用荧光定量PCR和蛋白免疫印迹技术检测患者鼻咽部抽取物、咽拭子和血清标本中的人博卡病毒DNA及特异性抗体,并全面分析感染患儿的各种临床症状、体征及常规实验室检测,同时做远期随访。结果240例急性下呼吸道感染患者的标本中检出人博卡病毒DNA阳性7例,阳性率为2.92%,其临床表现以高热、阵咳为主,实验室检查外周血白细胞、C-反应蛋白、血沉和血生化指标大多正常。X线胸片表现无特异性,远期随访心肺功能良好。结论人博卡病毒是引起小儿急性呼吸道感染的重要病原之一,荧光定量PCR和蛋白免疫印迹是灵敏、特异的检测技术。
Objectives To characterize the assay method and the clinical symptoms of the infant infection of the human bocavirus in order to advance the diagnositic and therapeutic technology for human bocavirus. Methods The DNA and specific antibody of human bocavirus from pharyngeal extracts, nasopharyngeal swab and serum were detected using real time PCR and Western blot. The overall diagnosis and analysis of the clinical symptoms, sign and conventional experimental test were performed, and meanwhile the long - term follow - up was done. Results 7 specimens were detected as human bocavirus positive from 240 pharyngeal extracts （7/240） , and the positive ratio was 2.92%. The clinical symptoms were mainly high fever and temporary cough, and the experimental index of peripheral blood leucocyte, C - reaction protein, blood sedimentation and hemobiochemistry were usually in normal region. No specific characteristic was observed by the thoracic X - ray. and cardio - pulmonary function was normal through the long - term investigation. Conclusions Human bocavirus was one of pathogens inducing children lower respiratory tract infections. The real time PCR and Western blot were sensitive and specific detection technologies.
出处
《医学研究杂志》
2006年第9期20-22,共3页
Journal of Medical Research
