摘要
目的采用HPLC法同时测定不同产地白芷中 2种香豆素的含量。方法色谱柱为岛津UL TRONVX ODS (2 5 0mm× 4 6mm ) ,5 μm柱 ,流动相为乙腈 水 (V∶V =6 5∶ 35 ) ,流速为0 8mL·min-1,检测波长 2 4 9nm。结果欧前胡素在 5～ 5 0mg·L-1(r =0 9999) ,异欧前胡素在2～ 2 0mg·L-1(r =0 9998)内线性关系良好 ,欧前胡素和异欧前胡素平均回收率分别为 98 4 %、96 3% ,RSD分别为 1 2 %、1 7%。结论不同产地白芷中 2种香豆素总量存在较大差异 。
Objective To determine the contents of two coumarins in Angelica dahurica from different regions by HPLC.Methods A ULTRON VX-ODS column(4.6 mm×250 mm,5 μm)was used with the mobile phase consisting of acetonitrile-water (V/∶V=65∶35,).The flow rate was 0.8 mL·min -1, and the detection wavelength was set at 249 nm.Results The linear ranges of imperatorin and isoimperatorin were 5～50 mg·L -1 (correlation coefficient r= 0.999 8) and 2～20 mg·L -1 (correlation coefficient r= 0.999 9),respectively.The average recoveries of imperatorin and isoimperatorin were 98.4%(RSD=1.2%) and 96.3% (RSD=1.7%), respectively.Conclusion There are great differences in the contents of two coumarines in Angelica dahurica from different regions.And the contents of two coumarines can provide a basis for the quality assessment for Angelica dahurica from different regions.
出处
《沈阳药科大学学报》
CAS
CSCD
2004年第5期354-357,共4页
Journal of Shenyang Pharmaceutical University
