摘要
建立了不同剂型保健品中类胡萝h素的提取方法和同时测定保健品中4种类胡萝h素含量的高效液相色谱分析方法。采用Diamonsil^TM C18色谱柱（250mm×4．6mmi．d．，5μm），以乙腈和乙酸乙酯为流动相进行梯度洗脱，洗脱程序：先以100％乙腈洗脱15min，然后乙酸乙酯的含量在10min内从0增加到100％，再以100％乙腈洗脱5min；流速1mL／min；采用二极管阵列检测器检测，检测波长为450nm。以外标法定量，番茄红素、叶黄素、玉米黄素、β-胡萝h素4种组分的线性关系良好，相关系数为0．9994～0．9997，检测限为0．4～0．5mg／L。片剂样品、粉状样品、油状样品的加标回收率分别为95．3％～98．7％，93．7％～98．8％，97．1％～99．2％，相对标准偏差（RSD）分别为0．89％～2．0％，0．89％-2．8％，0．42％1．2％。该法简便、快速、准确，是保健品中多种类胡萝卜素定量测定的可靠方法之一。
A method was established for the determination of carotenoids （lutein, zeaxanthin, lycopene, β-carotene） in health products by reversed-phase high performance liquid chromatography （RP-HPLC）. The extraction methods of carotenoids from different types of samples were also established. Separation and quantification was achieved by using a Diamonsil^TM C18 column （250 mm × 4.6 mm i. d. , 5 μm）, and a mobile phase of acetonitrile and ethyl acetate were used with the following gradient elution： 100% acetonitrile at the first 15 min, then ethyl acetate increased to 100% in the next 10 min, at last 100% acetonitrile for 5 min with a flow rate of 1 mL/min, temperature of 25 ℃ and monitored with a photodiode array detector at 450 nm. The external standard calibration curves were used for quantification. There were good linear correlations for the four carotenoids. The correlation coefficients were between 0.999 4 and 0.999 7. The detection limits were 0.4 -0.5 mg/L. The recoveries of four carotenoids in tablet samples were 95.3% - 98.7% with the relative standard deviations （RSDs） of 0.89% - 2.0%. The recoveries of four carotenoids in powder samples were 93.7% - 98.8% with RSDs of 0.89% -2.8%. The recoveries of four carotenoids in oil samples were 97. 1% -99.2% with RSDs of 0.42% - 1.2%. This method is simple, fast, accurate and convenient. It can be used as one of the reliable means for quantitative determination of carotenoid in health products.
出处
《色谱》
CAS
CSCD
北大核心
2006年第5期475-478,共4页
Chinese Journal of Chromatography
基金
国家科技攻关计划资助项目(No.2001BA804A21)
关键词
反相高效液相色谱
类胡萝卜素
番茄红素
叶黄素
玉米黄素
Β-胡萝卜素
保健品
reversed-phase high performance liquid chromatography （RP-HPLC）
carote-noids
lycopene
lutein
zeaxanthin
β-carotene
health products
