摘要
目的探讨恒磁场对血管内皮细胞活性、超微结构及凋亡的影响。方法将人脐静脉内皮细胞暴露于0.05、0.11、及5 mT恒磁场中,四唑盐比色法测定细胞增殖,采用透射电镜观察内皮细胞超微结构,流式细胞仪观测内皮细胞凋亡。结果0.05 mT恒磁场促进内皮细胞的增殖活性,0.1 mT恒磁场对细胞增殖无明显影响,1 mT、5mT恒磁场抑制细胞增殖。0.05 mT0、.1 mT1、mT恒磁场不引起细胞坏死,但1 mT恒磁场可引起部分细胞变性,5mT恒磁场不但引起细胞变性,还可以导致细胞坏死。0.05 mT、0.1 mT1、mT恒磁场没有引起细胞凋亡,5 mT恒磁场导致8.4%细胞凋亡。结论恒磁场对人脐静脉内皮细胞的生物学效应与磁感应强度有关,5 mT恒磁场对人脐静脉内皮细胞具有损害作用。
Aim To investigate the effects of static magnetic field on proliferation, ultra-microstructure, apoptosis of human umbilical vascular endothelial cells. Methods The effects of static magnetics on proliferation of human umbilical vascular endothelial cells was observed by MTT; transmission electric microscopy was used to evaluate the ultra-microstructure of human umbilical vascular endothelial cells; apoptosis of human umbilical vascular endothelial cells was studied by flow cytometry and transmission electric microscopy. Results 0.05 mT magnetics can accelerate the proliferation of human umbilical vascular endothelial cells; 0.1 mT magnetics have no effects on the growth of human umbilical vascular endothelial cells, however, magnetics of 1 mT, 5 mT attenuate growth of human umbilical vascular endothelial cells. 5 mT static magnetics could induce apoptosis and necrosis of human umbilical vascular endothelial cells. Conclusion The effects of static magnetics on human umbilical vascular endothelial cells is double edge-sword, 5 mT static magnetic field does harm to human umbilical vascular endothelial cells.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第2期140-142,共3页
Chinese Journal of Arteriosclerosis
