摘要
目的探讨肝星状细胞(HSC)激活与糖尿病树鼠句早期肝纤维化的关系。方法将树鼠句随机分为正常对照组和链脲佐菌素(STZ)处理组;静脉注射STZ,共持续8周给药,根据血糖水平,再分为糖尿病组(血糖≥11.1 mmol/L)和STZ对照组(血糖<11.1 mmol/L)。分别于成模前和成模后4、8周测定3组动物的血清ALT、AST和Ⅳ-C、LN、HA。8周末行肝组织活检,经HE、PAS、D-PAS、M asson染色及α-SMA免疫组化染色后光镜下观察。用病理图像分析系统对相关指标进行分析。以透射电镜观察肝超微结构。结果注射STZ 8周后糖尿病模型动物出现早期肝纤维化的电镜及光镜病理组织学变化,肝小叶内均出现激活的HSC,而正常和STZ对照组无一例出现激活的HSC;糖尿病组树鼠句肝组织α-SMA表达较其他两组高,α-SMA表达的面积密度与胶原纤维面积密度呈显著正相关。结论HSC激活可能在糖尿病早期肝纤维化的发生、发展中发挥重要作用。
To study the relationship between the activation of hepatic stellate cell （HSC） and the early stage of liver fibrosis in experimental diabetic tree shrews. Methods： Tree shrews were divided randomly into normal control group and streptozocin （STZ）-treated group. STZ-treated animals were then divided into diabetic group and STZ control group. ALT, AST, Type IV collagen （ IV-C）, Laminin （LN） and Hyaluronic acid （HA） were detected respectively before and after modelling. Liver biopsy was performed at the end of the experiment （ 8th week）. All of the liver specimens were observed in light microscope and transmission electron microscope, α-smooth muscle actin （α-SMA） was detected by immunohistochemistry and computer image analysis system. Resuits： Early fibrosis of the liver in diabetic tree shrews can be found by both light and electron microscope. There were some et-SMA positive HSCs, namely, activated HSCs could be observed in the liver of diabetic group but not in other two groups. The expression of α-SMA increased significantly in the liver of diabetic group as compared with that of other two groups. In addition, the level of expression of α-SMA was positively correlated with the collagen area density in diabetic group. Conclusion HSC may play an important role in the development and aggravation of liver fibrosis in diabetic tree shrews.
出处
《基础医学与临床》
CSCD
北大核心
2006年第6期621-625,共5页
Basic and Clinical Medicine
