摘要
目的探讨血清中游离的血红蛋白(不同程度溶血)对检测血清磷酸肌酸激酶(CK)活力的影响。方法用自制血红蛋白(Hb)液,制成不同浓度Hb含量的血清标本,用自动血细胞计数仪检测其Hb浓度,用自动生化仪配对检测原血清以及含不同浓度Hb血清的CK活力(U/L)。结果含不同浓度Hb血清比原血清CK活力均有不同程度的升高,且CK活力升高的程度与血清中Hb的浓度成正相关。结论在检测血清CK活力时,不宜采用溶血的血清标本,以免造成CK活力的假性增高。
Objective To evaluate the effect of the free haemoglobin （different concentration of haematolysis） on the detection of phosphocreatine kinase （CK） activity in serum.Methods Self-made Hb was added to serum specimens and the concentration of Hb was determined by the automatic blood cell meter. The CK activity in the original serum and the serum with different Hb content were detected by automatic biochemistry analyzer. Results The CK activity was higher in the Hb added serum with than in the original serum. The higher the haemoglobin concentration, the higher was the CK activity. Conclusion To avoid fale results of CK activity, specimens of haematolysis should not be used fro the detection of CK activity.
出处
《热带医学杂志》
CAS
2006年第5期563-564,共2页
Journal of Tropical Medicine
