摘要
利用PCR方法扩增获得罗氏沼虾(Macrobrachium rosenbergii)线粒体DNA的COⅠ基因,测定该基因片段序列。分析了罗氏沼虾缅甸原种F1代、江苏养殖群体和广西选育F2代3个群体共17只个体的序列核苷酸位点差异和遗传多态。结果表明,缅甸原种F1代遗传多样性最为丰富,江苏养殖群体和广西选育群体的遗传多样性相对贫乏。在长度为498 bp的基因片段中,共检测到10个多态性核苷酸位点(占2.01%),17只个体具有5种基因型,3群体各自的平均核苷酸位点差异分别为0.88%、0.07%和0。UPGMA分子系统聚类树显示,江苏养殖群体和广西选育群体的遗传关系最近,其单倍型混杂聚成一支,而缅甸原种F1群体相对独立为另外一支。COⅠ基因可以作为区分两分支群体的遗传标记。
The mitochondrial CO Ⅰ gene was PCR amplified and sequenced from 17 samples obtained from three populations of Mocrobrachium rosenbergii （F1 of the Burma wildtype population, Jiangsu cultured population and 1=2 of the Guangxi breeding population）. A 498 bp long partial gene segment was acquired and used to study the genetic diversity among the three populations. Results indicated that the CO Ⅰ gene locus was relatively more polymorphic in the F1 of Burma wildtype population, while the polymorphism in Jiangsu cultured population and Guangxi breeding population were very poor. A total of 10 polymorphic sites and 5 haplotypes were found in the sequences of the 17 samples. The average nucleotide divergence in the three populations was 0. 88 %, 0. 07 % and 0 respectively. The UPGMA phylogenetic tree suggested that F2 of the Guangxi breeding population and Jiangsu cultured population were closest genetically, and their heplotypes could be gathered together to a genetic branch; while F1 of the Burma wildtype population diverged and could form another relatively independent branch. For these distinct nucteotide differences, COⅠ gene could be suitable as a genetic marker for distinguishing these two branches of the Macrobrachium rosenbergii population.
出处
《遗传》
CAS
CSCD
北大核心
2006年第5期540-544,共5页
Hereditas（Beijing)
基金
广西科学基金项目(编号:0342006-1)资助~~
关键词
罗氏沼虾
CO
Ⅰ基因
序列多态性
遗传标记
Macrobrachium rosenbergii
CO Ⅰ gene
sequence polymorphism
genetic marker
