摘要
目的:探讨热休克蛋白27(heatshockprotein,HSP27)、bcl-2和p53蛋白在大肠癌组织中的表达及其临床意义。方法:运用免疫组织化学SP方法检测HSP27、bcl-2和p53在72例大肠癌组织中的表达,并运用多因素COX比例风险模型分析患者的预后。结果:HSP27在大肠癌组织中的阳性率为69·44%(50/72),bcl-2为54·17%(39/72),p53为59·72%(43/72)。HSP27在高分化腺癌组的表达率为84·85%,显著高于低分化组的28·57%,χ2=29·614,P=0·000。bcl-2和p53在高分化腺癌组的表达率也高于低分化腺癌组,χ2=5·771,P=0·016;χ2=4·714,P=0·030。HSP27和bcl-2在大肠癌中的表达具有相关性,r=0·380,P=0·001。COX回归分析提示,HSP27表达与生存期呈负相关。结论:HSP27和bcl-2及p53与大肠癌耐药有关;联合检测对临床制定合理的化疗方案具有指导意义;HSP27可作为判断大肠癌预后的一个独立预测指标。
OBJECTIVE： To evaluate the expressions and significance of HSP27, bcl-2 and p53 proteins in colorectal carcinoma. METHODS： The expressions of HSP27, bcl-2 and p53 in 72 cases of colorectal carcinoma were detected by SP immunohistochemical technique. RESULTS： The positive rates of HSP27, bcl-2 andp53 proteins were 69.44% （50/72）, 54.17% （39/72） and 59.72% （43/72）, respectively. The positive rate of HSP27 in the well differentiated group was 84.85% （28/33）, while it was 28. 57%（4/14） in the poor defferentiated group,x^2 =29. 614,P= 0. 000. The positive rates of hcl-2 and p53 were higher in the well differentiated group than those in the poor differentiated group x^2 =5. 771,P=0. 016; x^2 =4. 714,P=0. 030）. The expression of HSP27 was related to the expression of bcl-2, r= 0. 380, P = 0. 001. The correlation with prognosis was analyzed by COX proportional hazards model. It showed that HSP27 was independently associated with patient survival length. CONCLUSIONS： HSP27, bcl-2 and p53 are correlated with the multidrug resistance （MDR） of colorectal carcinoma. The unitive examination of HSP27,bcl-2 and p53 may help make out a chemotherapy plan. Expression of HSP27 protein is an independent and accurate predictor of poor clinical outcome for individual colorectal carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2006年第5期361-364,共4页
Chinese Journal of Cancer Prevention and Treatment
