摘要
本试验在农业部甘蔗生理生态与遗传改良重点开放实验室建立的甘蔗再生体系的基础上,在甘蔗愈伤组织培养的不同阶段,分别加入不同质量浓度的G418选择性试剂,以确定用于甘蔗遗传转化筛选的最适浓度.结果表明,G418对各供试甘蔗愈伤组织的抑制作用相似,愈伤组织生长、分化、生根3个阶段的抗性筛选质量浓度分别为20-35、15-35和25 mg.L-1.
The study was based on the regeneration system of sugarcane established by Key Lab of Eco-physiology ＆ Genetic Improvement for Sugarcane, Ministry of Agriculture. In the different stages of sugarcane callus culture, they were added into different concentration of G418, in order to ascertain the most suitable concentration of screening in sugarcane genetic transformation. The results showed that the restraining effects of G418 on each six sugarcane callus were similar. The screening concentration of G418 in the growth, regeneration and rootage stages of sugarcane callus were 20-35, 15-35 and 25 mg·L^-1 respectively.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2006年第1期6-10,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家"863"高新技术研究发展计划资助项目(2001AA241191
2002AA241031)
948引进国际先进技术项目(2003-Q06)资助
关键词
甘蔗愈伤组织
G418
筛选浓度
sugarcane callus
gencticin
screening concentration
