摘要
目的制备血管内皮生长因子反义寡核苷酸(VEGF-ASODN)的PLGA纳米粒,并探讨其制备工艺。方法采用复乳化溶剂挥发法制备,并用正交设计法对纳米粒的处方和制备工艺进行优化。结果纳米粒形态圆整,大小均匀,平均粒径150nm,包封率可达72%,含药量0.84%,体外释放缓慢,达到21天。结论VEGF-ASODN的PLGA纳米粒制备工艺简便、重现性好。
Objective To study vascular endothelial growth factor （VEGF） antisense oligodeoxynucleotide （VEGF- ASODN） loaded polylactic co-glyeolic acid （PLGA） nanoparticles and its formulation. Methods PLGA nanoparticles were prepared with w/o/w（water/oil/water） douhle emulsion-solvent evaporation method by orthogonal design. Results The shape of VEGF-ASODN loaded PLGA nanoparticles is roundness, size is well-distributed, the mean diameter is 150 nm, the encapsulation ratio reaches to 72%, the VEGF-ASODN loading in the nanoparticles is 0.84%, and the release is slow for at least 21 days. Conclusion The preparing method of VEGF-ASODN loaded PLGA nanoparticles is simple, and repeatability is fine.
出处
《中国介入影像与治疗学》
CSCD
2006年第1期55-58,共4页
Chinese Journal of Interventional Imaging and Therapy
基金
国家自然科学基金面上项目(30471993)
上海市科技发展基金(03ZR14030)
