摘要
背景与目的已有的研究表明,磁场能够引起水稻遗传特性的改变,其改变主要反映在水稻种子的萌发期、成长期、结实率等特性的变化,目前尚未见磁场对染色体形态结构、影响的研究,为此,我们分析不同磁场对水稻染色体的畸变,进一步探讨磁场的生物学机制。材料与方法采用800、1000、1500、1800、2300mT5种不同恒定磁场对水稻种子处理20min,采用ASG法对不同磁场处理后的水稻染色体进行染色,在光学显微镜下观察染色体畸变情况。结果除800mT的处理外,其他处理组水稻染色体均出现染色体环等畸变现象,其中包括无着丝粒、有着丝粒环及染色体间缺失后融合的倒“B”型染色体环,显示强磁场能引起染色体畸变率的提高,其主要为染色体断裂、融合现象出现率高于磁场影响的对照组水稻种子。结论磁场对染色体结构的影响是造成水稻遗传特色改变的重要原因之一。
BACKGROUDA＆AIM： Previous research its some hereditary characteristics such as germination, results showed that magnetic field treatment on rice can change maturation of seed and fructification rate etc, but no reports on chromosomal structure at present .We chose model plant,Oryza .sativa as materials for studing the mechanism of magnetic field effect on genetic characteristics. MATERIAL AND METHODS： After 800, 1 000, 1 500, 1 800 and 2 300 mT magnetic field treatments on Oryza.Sativa chromosom for 20 min, ASG method was adopted for chromosome staining, chromosome aberrations were examined with light microscope. RESULTS： Except 800 mT, some chromosomal aberrations were observed after 1 000, 1 500, 1 800 and 2 300 mT magnetic field treatment.These results showed that lasting magnetic field treatment could lead to chromosoal aberratinos such as no-centromere ,centromere-loop and inverse B-loop on chromosome, and provide biochemical evidence for the effects of magnetic field on chromosome. CONLUSION： Chromosomal aberrations caused by lasting magnetic field changed the genetic characteristics of O.sativa.
出处
《癌变．畸变．突变》
CAS
CSCD
2006年第1期29-31,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家民委自然科学基金重点项目(No.MZY020030)
湖北省自然科学基金资助项目(No.2002AB113)
关键词
磁场
水稻
畸变
magnetic field
Oryza. Sativa
aberration
