摘要
目的:探讨α-生育酚对精索静脉曲张(VC)大鼠睾丸生殖细胞损害的保护作用。方法:采用成年雄性Wistar大鼠10只复制精索静脉曲张疾病模型,4周后开始每日肌注α-生育酚0.5mg/(100g体重),共4周(生育酚组)。同时设手术组及假手术组进行对照。8周后用原位末端脱氧核苷酸转移酶标记法(TUNEL)检测睾丸生殖细胞凋亡指数(AI)。结果:生育酚组动物左侧睾丸重量显著高于手术组(P<0.05),低于假手术组(P<0.05);生育酚组细胞凋亡率[左侧(5.34±1.28)%,右侧(5.87±1.25)%]高于假手术组[左侧(4.72±1.02)%、右侧(4.88±1.28)%](P<0.05),而显著低于手术组[左侧(9.05±2.51)%,右侧(7.22±1.35)%](P<0.05)。结论:α-生育酚能降低精索静脉曲张大鼠生殖细胞的凋亡指数,延缓睾丸细胞损害的病程,对精索静脉曲张导致的睾丸损害具有一定的保护作用。
Objective：To study the effects of α-tocopherol on spermategenic cellular apoptosis in experimental varicocele in rats. Methods：Thirty adult male Wistar rats were randomized into 3 groups（ n =10）： treated group （TG）, operation group （OG）. and sham operation group （SOG）. Rats in treated group were injected intramusculously α-tocopherol （0.5 mg/100 g body-weight, one time daily for 4 weeks） after varicocele being induced by partial ligation of the left renal vein for 4 weeks. All the rats were breed in the similar condition for eight weeks. The apoptosis cells were detected by in situ terminal deoxynucleotityl transt＇erase mediated dTUP nick end lableling （TUNEL）. Results：The left vena spermatica of TG and OG were found cirsoid markedly comparing with SOG（ P 〈0.01）, the weight of left testicle in TG was intervened between the OG and SOG （ P 〈0.05）. The changes of histology were extra marked both in TG and OG, The apoptosis index of TG was lower than that of OG（ P 〈 0.05）, and higher than that of SOG（P〈 0.05）. Conelusions：h shows that α-tocopherol can decrease theapoptosis index in germ cells of varicocle rats, and slow down the proceeding of testicular injured.
出处
《临床泌尿外科杂志》
2006年第1期65-66,69,共3页
Journal of Clinical Urology
