摘要
目的探讨人精液抗氧化物尿酸与生殖细胞凋亡的关系。方法参照WHO标准方法，进行精液常规分析，按精子密度（×10^9／L）、活动率（％）不同分为4个组（正常、〈20、20～40、〉40）。采用尿酸酶一过氧化物酶偶联法检测精液尿酸含量。用脱氧核苷酸末端转移酶（TdT）介导的缺口末端标记（TUNEL）和瑞-姬染色法，分别检测和观察生殖细胞的凋亡。结果75例不育者精液尿酸含量和生殖细胞的凋亡率分别为163．37±57．15μmol／L和（16．38±1．25）％，与正常生育组397．60±52．1μmol／L、（4．61±1．23）％比较呈显著性差异（P〈0．01）。精子密度和活动率随精液尿酸含量减少而降低，生殖细胞凋亡率随之上升（P〈0．01）。不育组精液尿酸含量与生殖细胞的凋亡率呈显著性负相关（r=0．93。P〈0．05）。凋亡的生殖细胞体积缩小，核染色质致密，凝聚在核周围形成新月彤，或核裂解形成凋亡小体。结论精液尿酸含量与生殖细胞的凋亡有着密切关系。精液低尿酸含量时辛丸生殖细胞凋亡率高，精子密度和活率下降致男性不育。
Objective To explore the content of uric acid in semen and germ cell apoptosis in human. Methods Seminal fluid analysis was made according to WHO standard. There were four groups according to different density and motility rate of sperms （normal, 〈20, 20-40, 〉40）. The content of uric acid in semen was measured by uricase-peroxidase method. Using terminal deoxynueleotidyl transferase （TdT） mediated UTP nick end labeling （TUNEL） and Wright＇s Giemsa staining for the detection of germ cell apoptosis. Results In 75 infertile patients,the content of uric acid in semen were （163.37±57.15） μmol/L and germ cell apoptosis rate was （16.38±1.25）% ,which was significantly different from fertile group （397.60±52.1）μmol/L, （4.61± 1.23）%. Density and motility rate were decline accompany with the content of uric acid,and germ cell apoptosis rate ascend. The latter two were significantly negative related（r= 0. 93 ,P〈0.05）. The volume of apoptotic germ cell was decreased; chromatin was compact to form the cell-membrane blebs and apoptotic bodies. Conelusion Low concentration of uric acid can increase the frequency of apoptosis testis and the decline of density of sperm,whlch can include male infertility.
出处
《现代检验医学杂志》
CAS
2006年第1期23-25,共3页
Journal of Modern Laboratory Medicine
基金
第四军医大学西京高新技术基金资助(XJGX04045L11)
关键词
尿酸
生殖细胞
凋亡
精液
uric acid
germ cell
apoptosis
semen
