摘要
目的对福建省急性弛缓性麻痹（AFP）病例中分离的脊髓灰质炎病毒进行分子流行病学研究。方法用RT-PCR法扩增目的基因片段，通过核酸序列分析仪进行测序，将基因序列提交到美国国家生物技术信息中心（NCBI）GenBank上进行比对．并构建基因树。结果福建省1999～2002年分离到脊灰病毒5株，为Ⅱ型与Ⅲ型的疫苗重组。序列分析表明．福建省AFP病例中分离到的5株脊髓灰质炎病毒株VPI区与标准疫苗株的同源性高达99％以上．变异程度不大，与攀伎花市造成流行的脊灰病毒株差异也不大。同时发现，Fj-4，Fj-52株病毒为同一毒株。结论福建省在1999～2000年曾经出现过疫苗重组株，其中有一种毒株已在人群中循环，并引发2例病例。目前我国已进入无脊灰状态．仍采用减毒活疫苗进行免疫要注意脊髓灰质炎疫苗衍生株（VDPV）的流行。运用分子流行病学方法可以为适时调整免疫策略提供支持。
Objective To study the molecular epidemiology of poliovirus isolated from the feces of acute flaccid paralysis cases in Fujian. Methods The order genetic fragment was amplified by RT - PCR and then sequenced. The result of the virus sequence was given to NCBI Genebank for contrast, and constructed the phylongenetic trees. Results 5 poliovirus strains isolated in Fujian from 1999 to 2002 years, which were Sabinll and Sabin Ⅲ - recombinant strains. The sequence analysis showed the identities of these 5 poliovirus strains and standerd vaccine was over 99 %, it had seldom difference in nudetide, and was seldom difference from Panzhihua strains. Conclusion The vaccine - recombinant poliovirnses had even been in Fujian from 1999 to 2002, one of the virus strain had already circled in human, and caused two cases. At present, our nation had already been polio - free, we must pay attention to vaccine - derived polioviruses strains when we still rise OPV to immunize. The method of molecular epidemiology, can provide us support to adopt crrelation strategy to prevent and control polio in time.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第1期60-61,共2页
Chinese Journal of Public Health
基金
福建省卫生厅青年科研基金资助(2002-1-19)
