摘要
以牛肾细胞系(MDBK)培养牛传染性鼻气管炎病毒(IBRV)Bartha Nu/67株,经超速离心纯化病毒,再经超声破碎处理后作为诊断抗原,建立了检测牛血清IBRV抗体的间接酶联免疫吸附试验。该ELISA的判定标准为:血清D490 nm值大于0.369的判为阳性,小于0.295的判为阴性,在0.295与0.369之间的为可疑。特异性和重复性试验结果表明,该方法特异性高、重复性好。与法国进口ELISA抗体诊断试剂盒比较,其符合率为96.3%;与中和试验比较,符合率为95.8%,且敏感性更高。应用该诊断方法调查了我国部分地区IBRV的感染情况,结果显示,这些地区的IBRV感染率为67.1%。
An infectious bovine rhinotracheitis virus（IBRV） strain was cultured in Madin-Darby bovine kidney（MDBK） cells and purified by ultracentrifugation. The purified virus was disrupted by using an ultrasonic disintegrator. An indirect enzyme-linked immunosorbent assay（ELISA） was developed to detect specific antibodies to IBRV in serum with prepared diagnostic antigen. D490nm values higher than 0. 369 were determined as positive standard for the ELISA and lower than 0. 295 as negative standard. The assay was confirmed to be specific and reproducible. 96.3 % agreement was obtained by comparing to the IBR ELISA kit from France, and 95.8% by comparing to the virus neutralization test（VNT）. The IBRV infection in some areas of China was investigated by the established ELISA technique, and an average infection rate of 67.1% in cattle was obtained.
出处
《中国兽医科技》
CAS
CSCD
北大核心
2005年第12期959-963,共5页
Chinese Journal of Veterinary Science and Technology
基金
国家高技术研究发展计划(863)项目(2003AA241110)
黑龙江省科技攻关项目(GA02B501)
