摘要
根据伪狂犬病病毒(PRV)的gE基因序列,设计并合成了一对引物,以闽A株DNA为模板,建立了检测PRV的PCR方法。该方法能从猪细小病毒闽A株和FB株中扩增出一条长度为1 808 bp的片段,对Bartha株、gE-株检测为阴性;而以猪圆环病毒、猪繁殖与呼吸综合征病毒、猪伪狂犬病病毒、猪瘟病毒和正常细胞的核酸为模板的均为阴性;敏感性试验表明,该体系可检测到10pg的猪细小病毒基因组DNA。表明该方法适用于检测猪伪狂犬病病毒野毒株或非gE基因缺失弱毒株。
The PCR method to detect the pseudorabies virus （PRV） was established, after designed and synthesized of a pair of primers based on gE sequences of the PRV genome. This PCR technique was applied to specifically amplify the 1808 bp DNA fragment of the PRV Fa strains and FB strains. The negative results were achieved from Bartha-K61, gE^- strain, PK-15, porcine circovirus（PCV）, porcine reproductive and respiratory syndrome virus（PRRSV）, Porcine parvovirus（PPV）, Classical swine fever virus（CSFV）. The sensitivity of PCR reached to10 pg of PRV Fa strain DNA. The results showed that this PCR technique is reliable to detect wild strains and vaccine strain of PRV except gE^- strains of PRVs.
出处
《动物医学进展》
CSCD
2005年第11期81-83,共3页
Progress In Veterinary Medicine
