摘要
目的探讨肿瘤组织浸润树突状细胞(tumor infiltrating dendriticcells,TIDCs)在胃癌组织的表达及临床意义。方法采用免疫组化、原位杂交及流式细胞术分别检测4 5例胃癌组织的TIDCs的S1 0 0,CD 8 3及CD 8 3mRNA表达,并分析它们与临床病理特点的关系。结果免疫组化显示胃癌组织(4 2/4 5)中有S1 0 0+DCs不均一表达,CD 8 3表达于癌旁及正常黏膜。原位杂交显示CD 8 3mRNA有少量(7/4 5)表达。流式细胞术可检测到较低水平CD 8 3表达(4%)。S1 0 0和CD 8 3mRNA表达数量与患者年龄、性别、肿瘤部位、浸润深度、病理分期及淋巴结转移情况无显著相关性。免疫组化检测CD 8 3表达与TNM呈负相关(r=-0.9 2 4,P<0.0 1);流式细胞术检测CD 8 3与TNM分期及淋巴结转移呈负相关(r=-0.8 7 9,P<0.0 1;r=-0.7 8 2,P<0.0 5)。结论胃癌TIDCs以未成熟状态为主,S1 0 0表达提示其数量,CD 8 3表达提示其功能,且与胃癌TNM分期及淋巴结转移有关,是更好的临床观测指标。
Objective To investigate the expression and clinical significance of tumor infihrating dendritic cells （ TIDCs ） within gastric tumor tissues. Methods Immunohistochemistry （ IHC ） , in-situ hybridization （ ISH ） and flow cytometry were applied to detect the expression of S100, CD83 mRNA and CD83 on DCs in 45 gastric adenocarcinoma tissues. The co-relationship of the S100 and CD83 expression with clinical pathological features was analyzed. Results IHC showed that S100 expression was unevenly distributed within 42 cancer tissue and CD83 was only expressed in tumor-adjacent tissue and normal tissue. ISH showed that CD83 mRNA was limitedly expressed within 7 samples. S100 expression had no significant correlation with clinical pathological features, while CD83 reversely correlated with TNM stages. Detected by flow cytometry, CD83 was expressed in low level in all 45 gastric cancer tissues and negative correlations were found with lymph node metastasis and TNM stages of gastric cancer（ r = -0. 879, P 〈0.01; r = - 0. 782, P 〈 0.05 ） . Conclusions TIDCs in gastric cancer tissue mainly appear in an immature state. The expression of S 100 suggests the amount of TIDCs , while CD83 suggests their function. CD83 expression correlated with lymph node metastasis and TNM stages of gastric cancer, and could be used as a marker in further research.
出处
《中国普通外科杂志》
CAS
CSCD
2005年第10期740-743,共4页
China Journal of General Surgery
