摘要
目的研究血管内皮生长因子(VEGF)在上皮性卵巢癌组织中的表达状况并分析其与临床病理参数之间的关系。方法采用免疫组化、原位杂交技术分别检测31例上皮性卵巢癌、17例卵巢上皮性良性肿瘤及5例正常卵巢组织中VEGF蛋白质及核酸表达水平。结果上皮性卵巢癌组织中VEGF和VEGF mRNA阳性表达率分别为51.6%(16/31)和48.4%(15/31),与良性肿瘤相比有显著性差异(P<0.05)。上皮性卵巢癌中VEGF阳性表达率分别与淋巴结转移及腹水量密切相关(P<0.05),而在不同组织类型、病理分级及临床分期中无明显差异(P>0.05)。恶性肿瘤患者生存状况与癌组织中VEGF阳性表达率亦无相关性(P>0.05)。结论VEGF蛋白质及其mRNA在上皮性卵巢癌中高水平表达,且其阳性率与淋巴结转移及腹水量密切相关,可作为卵巢癌侵袭性评估的指标之一,为VEGF及其受体作为卵巢癌的治疗靶点提供科学依据。
Objective To investigate the relationship between the expressions of vascular endothelial growth factor （VEGF） protein and mRNA in human epithelial ovarian carcinoma and the pathological properties. Method The expressions of VEGF protein and mRNA were measured by immunohistochemical and in situ hybridizational techniques in 31 cases with human epithelial ovarian carcinoma and 17 with benign tumors and 5 with normal ovarian tissue. Results The positive expression rates of VEGF protein and mRNA in ovarian cancer were 51.6% （16/31） and 48.4% （15/31） respectively and were significantly higher than that in benign tumors（ P 〈 0.05）, There were significant associations between the expressions of VEGF and lymph node metastasis and the volume of ascites （ P 〈 0.05）. No correlations of VEGF expression were noted with historical types, pathological grades, clinical stage and prognosis （ P 〉 0.05）. Conclusion The expressions of VEGF and VEGF mRNA in epithelial ovarian carcinoma may enhance the predictability of patients at high risk for tumor progression as potential candidates for further aggressive therapy.
出处
《河南肿瘤学杂志》
2005年第5期310-312,共3页
Henan Journal of Oncology
关键词
卵巢肿瘤
血管内皮生长因子
肿瘤血管新生
ovarian neoplasm
vascular endothelial growth factors
tumor angiogenesis
