摘要
本研究证明冬虫夏草对体外培养的大鼠肾小管上皮细胞增殖具有明显促进作用，其增殖效应的机制可能与诱导肾小管细胞持续高水平地表达ｃ－ｍｙｃ原癌基因ｍＲＮＡ有关，鉴于急性肾衰治疗的中心环节正在于如何促进损伤、坏死的肾小管细胞再生、修复，因而推测虫草的上述作用可能对于急性肾衰治疗有益，采用虫草治疗庆大霉素诱发的大鼠急性肾损伤，确实具有良好的效果。
Cordyceps sinensis(CS)is a potent mitogen for spleen cell.We exami-ned the effect of CS on DNA synthesis in renal tubular cell,The effect of CS on tubu-lar cell expression Of mRNA encoding cell growth regulating protooncogene c-myc wasalso examined.￣3H-thymidine incorporation in tubular cell was stimulated after the abbit-ion of sera taken from rat fed with CS. Renal tubular cell responded to CS solutionwith a 2.2 fold increase in concentration of 50μg/ml.However,further increament of CSsolution to 75μg/ml induced an inhibition of cell proliferation.The mechanism of itsbiphasic effect remained unexplored.CS(5μg/ml)upregulated c-myc mRNA expressionwithin 30 minutes and the effect was substained for 4 hours,which suggested an inter-ference in cell growth by pathways associated with modulation of the c-myc mechanism. It was demonstrated previously that the adminstration of mitogen for renal tubularcell,like EGF and TGF-α,accelerated the regencration of tubular cell after acute tubu-lar injury.To investigate whether there exists an associated responsiveness in CS. westudied renal function and recovery of tubular damage in acute gentamicin nephrotoxicmodel of rat,The use of CS prompted an earlier recovery from acute tubular injury ascompard with control.Thus,both in vitro and in vivo studies proved that CS is amitogen for tubular cell and it may be a herb of value in treatment of acute renalfailure.
出处
《中国病理生理杂志》
CSCD
北大核心
1995年第2期211-215,共5页
Chinese Journal of Pathophysiology
