摘要
生物表面活性剂的研制是从本世纪70年代发展起来的。由于可产生表面活性剂的微生物种类、生物表面活性剂的类型及可作为其底物的原料较多,一些产品的性能与化学合成的相当,可降解性较大、比较安全,而日益受到注意。乙酸钙不动杆菌(Acinetobacter calcoaceticus)RAG-1产生的生物乳化剂Emulsan的商品化和生物表面活性剂在各种工业、尤其是石油工业中的可用性,也促进了该领域的发展。 糖脂是一类重要的生物表面活性剂。我们分离筛选到一株糖脂产生菌,鉴定为一新种:石蜡酪杆菌(caseobacter paraffinicnm)B126。本文报道该菌产生糖脂的适宜条件。
A suitable medium for the glycolipid synthesis by C. paraffinicum n. sP. B126 was elaborated using the orthogonal design of experiment, and composed of (g/L): NaNO3 1.5, K2HPO43H2O 1.0, KH2PO4 1.0, CaCl2 0.1, MgSO47H2O 0.5, yeast extract 2.0, EDTA 0.0015, n-hexadecane 10ml; pH 7.0-7.5. The inoculum age and amount were 48-72h and 5% (v/v) respectively. Cultures in 250 ml Erlenmeyer flasks containing 50 ml medium were incubated on a rotary shaker at 200 r/min. and 30c℃ for 50-60h. The surface and intersurface (against n-hexadecane) tension of the culture broth were 33 mN/m and 1-3 mN/m, respectively. The glycolipid yield was 3.7 g/L and 9 times higher than that in the primitive screening medium. The conversion rate of added n-hexadecane was 48 %.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第6期465-469,共5页
Acta Microbiologica Sinica
关键词
石蜡酪杆菌
糖脂
生物表面活性剂
Caseobacter paraffinicum B126, Suitable conditions for glycolipid pro- duction, Biosurfactant
